Expression analysis of LC3B and p62 indicates intact activated autophagy is associated with an unfavorable prognosis in colon cancer
| Autor: | Olivia Joan Adams, Julia Slotta-Huspenina, Franziska Graber, Sabina Berezowska, Rupert Langer, Inti Zlobec, Ulrich Nitsche, Robert D. Rosenberg, Mario P. Tschan, Monique Niklaus |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
autophagy Pathology medicine.medical_specialty Colorectal cancer 610 Medicine & health Biology Immunofluorescence 03 medical and health sciences 0302 clinical medicine LC3 medicine Tissue microarray medicine.diagnostic_test p62 Autophagy medicine.disease Staining 030104 developmental biology colon cancer Oncology Cell culture 030220 oncology & carcinogenesis immunohistochemistry Cancer research 570 Life sciences biology Immunohistochemistry Ex vivo Research Paper |
| Zdroj: | Niklaus, Monique; Adams, Olivia Joan; Berezowska, Sabina Anna; Zlobec, Inti; Graber, Franziska; Slotta-Huspenina, Julia; Nitsche, Ulrich; Rosenberg, Robert; Tschan, Mario; Langer, Rupert (2017). Expression analysis of LC3B and p62 indicates intact activated autophagy is associated with an unfavorable prognosis in colon cancer. OncoTarget, 8(33), pp. 54604-54615. Impact Journals LLC 10.18632/oncotarget.17554 Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.17554 |
| Popis: | Autophagy is a lysosomal degradation and recycling process implicated in cancer progression and therapy resistance. We assessed the impact of basal autophagy in colon cancer (CC) in vitro and ex vivo. Functional autophagy was demonstrated in CC cell lines (LoVo; HT-29) showing a dose-dependent increase of the autophagy markers LC3B, p62 and autophagic vesciles upon increasing concentrations of the autophagy inhibitor chloroquine, which was demonstrated by immunoblotting, immunofluorescence and electron microscopy. Next, tissue microarrays with 292 primary resected CC, with cores from different tumor regions, and normal mucosa were analyzed by immunohistochemistry for LC3B and p62. CC tissue showed LC3B dot-like, p62 dot-like, cytoplasmic and nuclear staining in various levels without significant intratumoral heterogeneity. Tumoral LC3B and p62 expression was significantly higher than in normal tissue (p |
| Databáze: | OpenAIRE |
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