Role of Lymphotoxin-α Gene Polymorphism in Hepatitis C Virus-Related Chronic Liver Disorders
| Autor: | Amr Mohamed Zaghloul, Hammam Tammam, Ghada Moustafa Galal, Abeer Sheneef, Nagwa S. Ahmed, Amal M Abdel Aal, Nahed Fathallah Fahmy |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lymphotoxin alpha hepatitis C virus medicine.medical_specialty Cirrhosis gene polymorphism Hepatitis C virus 030106 microbiology medicine.disease_cause Gastroenterology 03 medical and health sciences Liver disease 0302 clinical medicine Polymorphism (computer science) Internal medicine Genotype medicine Pharmacology (medical) 030212 general & internal medicine Original Research Pharmacology business.industry hepatocellular carcinoma medicine.disease digestive system diseases Infectious Diseases Infection and Drug Resistance Hepatocellular carcinoma Gene polymorphism lymphotoxin alpha business |
| Zdroj: | Infection and Drug Resistance |
| ISSN: | 1178-6973 |
| Popis: | Ghada Galal,1 Hammam Tammam,1 Amal Abdel Aal,2 Nahed Fahmy,3 Abeer Sheneef,3 Nagwa Ahmed,4 Amr Zaghloul1 1Department of Tropical Medicine and Gastroenterology, Sohag University, Sohag, Egypt; 2Department of Clinical Pathology, Assiut University, Assiut, Egypt; 3Department of Medical Microbiology and Immunology, Sohag University, Sohag, Egypt; 4Department of Biochemistry, Sohag University, Sohag, EgyptCorrespondence: Amr Zaghloul Tel +201006523309Email amrmoh_80@yahoo.comBackground: Tumor necrosis factor (TNF) family includes lymphotoxin-alpha (LTA) which is a pro-inflammatory cytokine which plays a role in hepatic fibrogenesis. LTA gene polymorphism plays a role in different inflammatory and immunomodulatory diseases. This polymorphism is also suggested to affect chronic hepatitis C (CHC) infection course.Aim: To study the contribution of LTA gene polymorphism in different chronic hepatitis C stages and hepatocellular carcinoma risk.Patients and Methods: Our study included 108 chronic HCV patients grouped according to the disease stage. Group (A): CHC, group (B): liver cirrhosis (LC), group (C): LC with HCC, and group (D): healthy controls. Routine laboratory investigations, polymerase chain reaction (PCR) for quantification of HCV, abdominal ultrasonography, and Liver stiffness measurement (LSM) were done. Child–Turcotte–Pugh, Model for end-stage liver disease (MELD), and Fibrosis index based on 4 (FIB-4) scores were calculated. We used the PCR-restriction fragment length polymorphism technique for lymphotoxin-α genotyping.Results: The A/G genotype was predominant in all groups. In HCC patients, G/G genotype was more frequent (31.8%) than in the LC group (19.4%), CHC group (17.8%), and controls (4.17%). A significant association was found between LTA genotypes and the child classes in HCC (P< 0.01) but not in LC patients (P> 0.05). HCC patients carrying A/G genotype had higher MELD scores than other genotypes. Multivariate binary logistic regression analysis confirmed that LTA G/G genotype and low platelet count were independent predictors for HCC development in patients with HCV-related LC.Conclusion: Detection of LTA G/G genotype in chronic HCV patients could help to recognize high-risk patients for disease progression and HCC development.Keywords: hepatitis C virus, lymphotoxin alpha, hepatocellular carcinoma, gene polymorphism |
| Databáze: | OpenAIRE |
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