Genomic analysis of 600 vancomycin-resistant Enterococcus faecium reveals a high prevalence of ST80 and spread of similar vanA regions via IS1216E and plasmid transfer in diverse genetic lineages in Ireland
| Autor: | Henrik Westh, Gráinne I. Brennan, Brian O'Connell, Anna C. Shore, Sarah Mollerup, Brenda A. McManus, David C. Coleman, José Alfredo Samaniego Castruita, Sarah A Egan, Mette Pinholt, Nicole L Kavanagh |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
Transposable element Enterococcus faecium Vancomycin-Resistant Enterococci Plasmid Vancomycin Prevalence Humans AcademicSubjects/MED00740 Pharmacology (medical) Allele Clade Gram-Positive Bacterial Infections Transposase Original Research Vancomycin resistant Enterococcus faecium Pharmacology Genetics Cross Infection biology Genomics biochemical phenomena metabolism and nutrition biology.organism_classification AcademicSubjects/MED00290 Infectious Diseases Multilocus sequence typing AcademicSubjects/MED00230 Ireland Multilocus Sequence Typing Plasmids |
| Zdroj: | Egan, S A, Kavanagh, N L, Shore, A C, Mollerup, S, Samaniego Castruita, J A, O’connell, B, Mcmanus, B A, Brennan, G I, Pinholt, M, Westh, H & Coleman, D C 2022, ' Genomic analysis of 600 vancomycin-resistant Enterococcus faecium reveals a high prevalence of ST80 and spread of similar vanA regions via IS1216E and plasmid transfer in diverse genetic lineages in Ireland ', Journal of Antimicrobial Chemotherapy, vol. 77, no. 2, pp. 320-330 . https://doi.org/10.1093/jac/dkab393 Journal of Antimicrobial Chemotherapy |
| Popis: | Background Vancomycin-resistant Enterococcus faecium (VREfm) cause a wide range of hospital infections. Ireland has had one of the highest invasive VREfm infection rates in Europe over the last decade, yet little is known about Irish VREfm. Objectives To investigate the population structure of Irish VREfm, explore diversity by analysing the vanA transposon region and compare Irish, Danish and global isolates. Methods E. faecium (n = 648) from five Irish hospitals were investigated, including VREfm [547 rectal screening and 53 bloodstream infection (BSI)] isolates and 48 vancomycin-susceptible (VSEfm) BSI isolates recovered between June 2017 and December 2019. WGS and core-genome MLST (cgMLST) were used to assess population structure. Genetic environments surrounding vanA were resolved by hybrid assembly of short-read (Illumina) and long-read (Oxford Nanopore Technologies) sequences. Results All isolates belonged to hospital-adapted clade A1 and the majority (435/648) belonged to MLST ST80. The population structure was highly polyclonal; cgMLST segregated 603/648 isolates into 51 clusters containing mixtures of screening and BSI isolates, isolates from different hospitals, and VREfm and VSEfm. Isolates within clusters were closely related (mean average ≤16 allelic differences). The majority (96.5%) of VREfm harboured highly similar vanA regions located on circular or linear plasmids with multiple IS1216E insertions, variable organization of vanA operon genes and 78.6% harboured a truncated tnpA transposase. Comparison of 648 Irish isolates with 846 global E. faecium from 30 countries using cgMLST revealed little overlap. Conclusions Irish VREfm are polyclonal, yet harbour a characteristic plasmid-located vanA region with multiple IS1216E insertions that may facilitate spread. |
| Databáze: | OpenAIRE |
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