Pure Δ 9 -tetrahydrocannabivarin and a Cannabis sativa extract with high content in Δ 9 -tetrahydrocannabivarin inhibit nitrite production in murine peritoneal macrophages
| Autor: | Francesca Borrelli, Maria Grazia Cascio, Vincenzo Di Marzo, Pierangelo Orlando, Angelo A. Izzo, Roger G. Pertwee, Ester Pagano, Raffaele Capasso, Barbara Romano |
|---|---|
| Přispěvatelé: | Romano, Barbara, Pagano, Ester, Orlando, Pierangelo, Capasso, Raffaele, Cascio, Maria Grazia, Pertwee, Roger, Marzo, Vincenzo Di, Izzo, ANGELO ANTONIO, Borrelli, Francesca |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cannabinoid receptor medicine.medical_treatment Pharmacology Tetrahydrocannabivarin Nitric oxide 03 medical and health sciences Transient receptor potential channel chemistry.chemical_compound Internal medicine medicine Cannabinoid receptor type 2 Cannabi Cannabinoid receptors Receptor Transient receptor potential (TRP) channels Cannabis Transient receptor potential (TRP) channel Inflammation biology Nitric oxide synthase 030104 developmental biology Endocrinology chemistry biology.protein lipids (amino acids peptides and proteins) Phytocannabinoid Cannabinoid Phytocannabinoids |
| Zdroj: | Pharmacological research (Online) 113 (2016): 199–208. doi:10.1016/j.phrs.2016.07.045 info:cnr-pdr/source/autori:Barbara Romanoa, Ester Paganoa, Pierangelo Orlando, Raffaele Capassoa, Maria Grazia Cascio, Roger Pertwee, Vincenzo Di Marzo, Angelo A. Izzoa, Francesca Borrelli/titolo:Pure delta9-tetrahydrocannabivarin and a Cannabis sativa extract withhigh content in delta9-tetrahydrocannabivarin inhibit nitrite productionin murine peritoneal macrophages/doi:10.1016%2Fj.phrs.2016.07.045/rivista:Pharmacological research (Online)/anno:2016/pagina_da:199/pagina_a:208/intervallo_pagine:199–208/volume:113 |
| ISSN: | 1043-6618 |
| DOI: | 10.1016/j.phrs.2016.07.045 |
| Popis: | Historical and scientific evidence suggests that Cannabis use has immunomodulatory and anti-inflammatory effects. We have here investigated the effect of the non-psychotropic phytocannabinoid Δ9-tetrahydrocannabivarin (THCV) and of a Cannabis sativa extract with high (64.8%) content in THCV (THCV-BDS) on nitric oxide (NO) production, and on cannabinoid and transient receptor potential (TRP) channel expression in lipopolysaccharide (LPS)-stimulated murine peritoneal macrophages. THCV-BDS and THCV exhibited similar affinity in radioligand binding assays for CB1 and CB2 receptors, and inhibited, via CB2 but not CB1 cannabinoid receptors, nitrite production evoked by LPS in peritoneal macrophages. THCV down-regulated the over-expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and interleukin 1β (IL-1β) proteins induced by LPS. Furthermore, THCV counteracted LPS-induced up-regulation of CB1 receptors, without affecting the changes in CB2, TRPV2 or TRPV4 mRNA expression caused by LPS. Other TRP channels, namely, TRPA1, TRPV1, TRPV3 and TRPM8 were poorly expressed or undetectable in both unstimulated and LPS-challenged macrophages. It is concluded that THCV - via CB2 receptor activation - inhibits nitrite production in macrophages. The effect of this phytocannabinoid was associated with a down-regulation of CB1, but not CB2 or TRP channel mRNA expression. |
| Databáze: | OpenAIRE |
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