Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?
| Autor: | Cristina Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, María Cristina Manzanares-Cespedes, Matthias Widbiller |
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| Rok vydání: | 2022 |
| Předmět: |
610 Medizin
Stem cells Catalysis Endodontics Inorganic Chemistry Polpa dental stomatognathic system Osteogenesis human amnion epithelial cells dental pulp stem cells dentin matrix proteins odontoblastic differentiation revitalization Humans Regeneration ddc:610 Amnion Endodòncia Physical and Theoretical Chemistry Molecular Biology Spectroscopy Cells Cultured Dental Pulp Cell Proliferation Stem Cells Organic Chemistry Cell Differentiation Epithelial Cells General Medicine Computer Science Applications Dental pulp Dentin cardiovascular system Dentina Cèl·lules mare |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona International Journal of Molecular Sciences; Volume 23; Issue 5; Pages: 2830 |
| Popis: | The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial–mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype; however, genes associated with epithelial–mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition. |
| Databáze: | OpenAIRE |
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