Large-Scale Purification of Photon-Upconversion Nanoparticles by Gel Electrophoresis for Analogue and Digital Bioassays
| Autor: | A. Król, Antonín Hlaváček, Hans H. Gorris, Matthias Jürgen Mickert, Naděžda Pizúrová, Terhi Tallgren, Satu Lahtinen, Tero Soukka |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Streptavidin
Bioanalysis Infrared Rays Dispersity Nanoparticle Biotin Metal Nanoparticles 010402 general chemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Europium Particle Size ta116 Gel electrophoresis Electrophoresis Agar Gel Chromatography Luminescent Agents 010401 analytical chemistry Silicon Dioxide Photon upconversion 0104 chemical sciences Electrophoresis chemistry Nanoconjugates |
| Zdroj: | Analytical chemistry. 91(2) |
| ISSN: | 1520-6882 |
| Popis: | The performance of photon-upconversion nanoparticles (UCNPs) as background-free luminescent labels in bioanalytical applications strongly depends on the preparation of well-defined and water-dispersible nanoconjugates. We have exploited the separation power of agarose-gel electrophoresis to purify milligram amounts of homogeneous UCNPs covered with carboxylated silica, biotin, or streptavidin with recovery rates of 30 to 50%. Clusters containing discrete numbers of UCNPs were isolated from the gel and reanalyzed by agarose-gel electrophoresis, single-nanoparticle-upconversion microscopy, and additional complementary methods. The purified nanoconjugates improved conventional (analogue) bioaffinity assays and provided highly monodisperse conjugates for assays that rely on counting individual UCNPs (digital assays). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|