Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase
| Autor: | Elizabeth B. Weiler, Bryan W. Berger, Logan C. MacDonald |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Glycan Stenotrophomonas maltophilia Uronic acid Management Monitoring Policy and Law Polysaccharide 7. Clean energy Applied Microbiology and Biotechnology Alginate lyase 03 medical and health sciences chemistry.chemical_compound Hydrolysis chemistry.chemical_classification 030102 biochemistry & molecular biology biology Renewable Energy Sustainability and the Environment Research Alginate Carbohydrate biology.organism_classification Brown algae 030104 developmental biology General Energy Enzyme chemistry Biochemistry biology.protein Digestion Biotechnology |
| Zdroj: | Biotechnology for Biofuels |
| ISSN: | 1754-6834 |
| DOI: | 10.1186/s13068-016-0455-8 |
| Popis: | Background Macroalgae represents a promising source of fermentable carbohydrates for use in the production of energy efficient biofuel. The primary carbohydrate in brown algae is the uronic acid-containing alginate, whereas green algae contains a significant amount of glucuronan. A necessary step in the conversion of these polyuronides to bioethanol is saccharification, which can be achieved by enzymatic or chemical degradation. Results Polysaccharide lyases are a class of enzymes which cleave uronic acid-containing glycans via a β-elimination mechanism, acting both endo- and exolytically on their substrates. In the present work, we characterize a putative alginate lyase from Stenotrophomonas maltophilia K279a (Smlt2602) and describe a H208F mutant that, in addition to cleaving alginate-based substrates, displays significant, exolytic glucuronan activity. Conclusions To our knowledge this is the first polysaccharide lyase to act exolytically on glucuronan and is an attractive candidate for the broad-spectrum digestion of polyuronides into fermentable monomers. Electronic supplementary material The online version of this article (doi:10.1186/s13068-016-0455-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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