Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
| Autor: | Chun-Wei Chang, Wen-I Luo, Huai-Chun Chuang, Jen-Lin Chang, Yi-Fang Tsai, Guor-Tzo Wei, Ravirala Ramu, Steve S.-F. Yu, Jyh-Myng Zen |
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| Rok vydání: | 2017 |
| Předmět: |
Stereochemistry
Science AlkB Hydroxylation 010402 general chemistry 01 natural sciences Redox Article Catalysis Adduct chemistry.chemical_compound Propane Rubredoxin Alkanes Multidisciplinary biology Pseudomonas putida 010405 organic chemistry Chemistry Rubredoxins Electrochemical Techniques biology.organism_classification 0104 chemical sciences X-Ray Absorption Spectroscopy Biochemistry Alcohols biology.protein Medicine Cytochrome P-450 CYP4A |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| Popis: | An unprecedented method for the efficient conversion of C3–C12 linear alkanes to their corresponding primary alcohols mediated by the membrane-bound alkane hydroxylase (AlkB) from Pseudomonas putida GPo1 is demonstrated. The X-ray absorption spectroscopy (XAS) studies support that electrons can be transferred from the reduced AlkG (rubredoxin-2, the redox partner of AlkB) to AlkB in a two-phase manner. Based on this observation, an approach for the electrocatalytic conversion from alkanes to alcohols mediated by AlkB using an AlkG immobilized screen-printed carbon electrode (SPCE) is developed. The framework distortion of AlkB–AlkG adduct on SPCE surface might create promiscuity toward gaseous substrates. Hence, small alkanes including propane and n-butane can be accommodated in the hydrophobic pocket of AlkB for C–H bond activation. The proof of concept herein advances the development of artificial C–H bond activation catalysts. |
| Databáze: | OpenAIRE |
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