Transferability of nuclear microsatellite markers to the atmospheric bromeliads Tillandsia recurvata and T. aeranthos (Bromeliaceae)
| Autor: | Felipe Aoki-Gonçalves, Bárbara Simões Santos Leal, Davi Rodrigo Rossatto, Clarisse Palma-Silva, Cleber Juliano Neves Chaves |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Estadual de Campinas (UNICAMP) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
SSR markers biology Tillandsia Range (biology) Ecology Population genetics Bromeliaceae Plant Science biology.organism_classification 010603 evolutionary biology 01 natural sciences Cross-amplification Abundance (ecology) Tillandsia recurvata Epiphyte Species richness Epiphytes 010606 plant biology & botany |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2019-10-06T16:57:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The genus Tillandsia L. is the most diversified genus of Bromeliaceae and represents one of the most specialized cases of vascular epiphytism: the ‘atmospheric bromeliads.’ Such great diversity and ecological specialization make it an interesting model for evolutionary and population genetics studies. Here we report the cross-transferability of SSR markers isolated from other bromeliad species to Tillandsia recurvata (L.) L. and T. aeranthos (Loisel.) Desf., epiphytes with great abundance in both natural and anthropogenic-modified environments, but with contrasting patterns of geographic distribution and mating systems. We tested a total of 27 microsatellite markers and successfully amplified seven polymorphic markers in T. recurvata and T. aeranthos. We then described cross-amplified markers in two populations per species, sampled in both anthropogenic-transformed and natural environments. T. recurvata presented lower allelic richness and heterozygosities, and greater inbreeding coefficient values. Such differences clearly reflect their contrasting mating systems (self-fertilizing in T. recurvata versus self-incompatible in T. aeranthus). The set of cross-amplified microsatellite markers described here will be a helpful tool to address a range of evolutionary and ecological questions. Programa de Pós-graduação em Ecologia e Biodiversidade Instituto de Biociências Universidade Estadual Paulista (Unesp) Departamento de Biologia Universidade Estadual Paulista (Unesp) Departamento de Biologia Vegetal Instituto de Biologia Universidade Estadual de Campinas Programa de Pós-graduação em Ecologia e Biodiversidade Instituto de Biociências Universidade Estadual Paulista (Unesp) Departamento de Biologia Universidade Estadual Paulista (Unesp) FAPESP: 2014/08087-0 FAPESP: 2016/03777-4 FAPESP: 2016/04396-4 CNPq: 300819/2016-1 CNPq: 471756/2013-0 FAPESP: FAPESP 2014/15588-6 |
| Databáze: | OpenAIRE |
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