Protective Effect of Catechin on Apoptosis of the Lens Epithelium in Rats with N-methyl-N-nitrosourea-induced Cataracts
| Autor: | Dong-Hee Kim, Byung Nam Kang, Sung Eun Kim, Sung Min Lee, Il-Gyu Ko |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
genetic structures
medicine.medical_treatment Blotting Western Intraperitoneal injection Apoptosis Caspase 3 Epithelial cells Cataract Catechin Immunoenzyme Techniques Rats Sprague-Dawley Andrology Random Allocation chemistry.chemical_compound Cataracts In Situ Nick-End Labeling Lens Crystalline medicine Animals Crystalline lens Analysis of Variance TUNEL assay business.industry General Medicine medicine.disease eye diseases Rats Animals Newborn chemistry Biochemistry Immunohistochemistry Original Article sense organs business |
| Zdroj: | Korean Journal of Ophthalmology : KJO |
| ISSN: | 1011-8942 |
| Popis: | Purpose: To investigate the effect of catechin on apoptotic cell death in the lens epithelium of rats with cataract. Methods: Cataract was induced by intraperitoneal injection of 100 mg/kg N-methyl-N-nitrosourea (MNU) to ten day-old Sprague-Dawley rats. The neonatal rats were randomly divided into five groups (n=15 in each group): a control group, and four cataract-induction groups, treated with either 0, 50, 100, 200 mg/kg catechin. We performed slit-lamp biomicroscopic analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, Western-blot for Bcl-2 and Bax, and immunohistochemistry for caspase-3. Results: Apoptotic cell death in lens epithelial cells that increased following cataract formation in rats was suppressed by cathechin. Conclusions: Catechin inhibited cataract-induced apoptotic cell death in the lens epithelium and may prove useful for the prevention of cataract progression. |
| Databáze: | OpenAIRE |
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