Characterization of phenolic compounds in green and red oak-leaf lettuce cultivars by UHPLC-DAD-ESI-QToF/MS using MSEscan mode
| Autor: | Sara Ines Roura, Gabriela Elena Viacava, C. Iriondo, Rosa M. Alonso-Salces, Luis A. Berrueta, Blanca Gallo |
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| Rok vydání: | 2017 |
| Předmět: |
MASS SPECTROMETRY
Spectrometry Mass Electrospray Ionization Electrospray ionization Analytical chemistry Lactuca Mass spectrometry 01 natural sciences chemistry.chemical_compound 0404 agricultural biotechnology Glucosides Phenols Glucoside POLYPHENOL Cultivar Apigenin Luteolin Chromatography High Pressure Liquid Spectroscopy Chromatography Molecular Structure biology Plant Extracts Otras Ciencias Químicas 010401 analytical chemistry Ciencias Químicas 04 agricultural and veterinary sciences Lettuce biology.organism_classification 040401 food science 0104 chemical sciences Plant Leaves COUMARIN chemistry Polyphenol Anthocyanin LIGNAN ANTHOCYANIN Monoisotopic mass LACTUCA SATIVA CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Journal of Mass Spectrometry. 52:873-902 |
| ISSN: | 1076-5174 |
| DOI: | 10.1002/jms.4021 |
| Popis: | Lettuce (Lactuca sativa) is one of the most popular leafy vegetables in the world and constitutes a major dietary source of phenolic compounds with health-promoting properties. In particular, the demand for green and red oak-leaf lettuces has considerably increased in the last years but few data on their polyphenol composition are available. Moreover, the usage of analytical edge technology can provide new structural information and allow the identification of unknown polyphenols. In the present study, the phenolic profiles of green and red oak-leaf lettuce cultivars were exhaustively characterized by ultrahigh-performance liquid chromatography (UHPLC) coupled online to diode array detection (DAD), electrospray ionization (ESI), and quadrupole time-of-flight mass spectrometry (QToF/MS), using the MSE instrument acquisition mode for recording simultaneously exact masses of precursor and fragment ions. One hundred fifteen phenolic compounds were identified in the acidified hydromethanolic extract of freeze-dried lettuce leaves. Forty-eight of these compounds were tentatively identified for the first time in lettuce, and only 20 of them have been previously reported in oak-leaf lettuce cultivars in literature. Both oak-leaf lettuce cultivars presented similar phenolic composition, except for apigenin-glucuronide and dihydroxybenzoic acid, only detected in the green cultivar; and for luteolin-hydroxymalonylhexoside, an apigenin conjugate with molecular formula C40H54O19 (monoisotopic MW = 838.3259 u), cyanidin-3-O-glucoside, cyanidin-3-O-(3″-O-malonyl)glucoside, cyanidin-3-O-(6″-O-malonyl)glucoside, and cyanidin-3-O-(6″-O-acetyl)glucoside, only found in the red cultivar. The UHPLC-DAD-ESI-QToF/MSE approach demonstrated to be a useful tool for the characterization of phenolic compounds in complex plant matrices. Fil: Viacava, Gabriela Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Investigación en Ingeniería en Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Roura, Sara Ines. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Investigación en Ingeniería en Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Berrueta, Luis A.. Universidad del País Vasco; España Fil: Iriondo, Carmen. Universidad del País Vasco; España Fil: Gallo, Blanca. Universidad del País Vasco; España Fil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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