Location of transient ectodermal progenitor potential in mouse development
| Autor: | Fredrik Lanner, Qingqing Zhu, Naihe Jing, Lingyu Li, Chang Liu, Janet Rossant, Lu Song, Steffen Biechele |
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| Rok vydání: | 2013 |
| Předmět: |
Pluripotent Stem Cells
animal structures Nodal Protein Embryonic Development Nodal signaling Ectoderm Bone Morphogenetic Protein 4 Germ layer Biology Histogenesis Models Biological Somatopleuric mesenchyme Mice medicine Animals Cell Lineage Molecular Biology Genetics Mice Inbred ICR Stem Cells Embryogenesis Diploblasty Gene Expression Regulation Developmental Embryo Mammalian Cell biology medicine.anatomical_structure Epidermal Cells Epiblast embryonic structures Biomarkers Signal Transduction Developmental Biology |
| Zdroj: | Development. 140:4533-4543 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.092866 |
| Popis: | Ectoderm is one of the three classic germ layers in the early mouse embryo, with the capacity to develop into both the central nervous system and epidermis. Because it is a transient phase of development with few molecular markers, the early ectoderm is the least understood germ layer in mouse embryonic development. In this work, we studied the differentiation potential of isolated ectoderm tissue in response to BMP signaling at various developmental stages (E6.5, E7.0 and E7.5), and identified a transient region in the anterior-proximal side of the embryo at E7.0 that possesses the ability to become neural or epidermal ectoderm in response to the absence or presence of BMP4, respectively. Furthermore, we demonstrated that inhibition of Nodal signaling could direct the pluripotent E6.5 epiblast cells towards ectoderm lineages during differentiation in explants in vitro. Our work not only improves our understanding of ectodermal layer development in early embryos, but also provides a framework for regenerative differentiation towards ectodermal tissues. |
| Databáze: | OpenAIRE |
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