Transmembrane Coordination of Preprotein Recognition and Motor Coupling by the Mitochondrial Presequence Receptor Tim50
| Autor: | Raffaele Ieva, Martin van der Laan, Bernard Guiard, Anne Caumont-Sarcos, Cyril Moulin, Lucyle Poinot |
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| Přispěvatelé: | Laboratoire de microbiologie et génétique moléculaires (LMGM), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins [SDV]Life Sciences [q-bio] Saccharomyces cerevisiae Mitochondrion Mitochondrial Membrane Transport Proteins Models Biological General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Protein Domains Mitochondrial Precursor Protein Import Complex Proteins Translocase Inner membrane Inner mitochondrial membrane lcsh:QH301-705.5 biology Chemistry Molecular Motor Proteins Cell Membrane Transmembrane protein Cell biology Mitochondria Coupling (electronics) Transmembrane domain Protein Transport 030104 developmental biology lcsh:Biology (General) biology.protein Intermembrane space 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports Cell Reports, Elsevier Inc, 2020, 30, pp.3092-3104.e4. ⟨10.1016/j.celrep.2020.02.031⟩ Cell Reports, Vol 30, Iss 9, Pp 3092-3104.e4 (2020) |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2020.02.031⟩ |
| Popis: | Summary: Mitochondrial preproteins contain amino-terminal presequences directing them to the presequence translocase of the mitochondrial inner membrane (TIM23 complex). Depending on additional downstream import signals, TIM23 either inserts preproteins into the inner membrane or translocates them into the matrix. Matrix import requires the coupling of the presequence translocase-associated motor (PAM) to TIM23. The molecular mechanisms coordinating preprotein recognition by TIM23 in the intermembrane space (IMS) with PAM activation in the matrix are unknown. Here we show that subsequent to presequence recognition in the IMS, the Tim50 matrix domain facilitates the recruitment of the coupling factor Pam17. Next, the IMS domain of Tim50 promotes PAM recruitment to TIM23. Finally, the Tim50 transmembrane segment stimulates the matrix-directed import-driving force exerted by PAM. We propose that recognition of preprotein segments in the IMS and transfer of signal information across the inner membrane by Tim50 determine import motor activation. : Caumont-Sarcos et al. report a transmembrane coordination mechanism that promotes import of presequence-containing preproteins into mitochondria. The presequence translocase subunit Tim50 links the preprotein receptor function on the cis side of the mitochondrial inner membrane to functional activation of the import motor on the trans side. Keywords: mitochondria, preprotein import, presequence translocase, import motor, TIM23, Tim50, mitochondrial Hsp70, Pam17 |
| Databáze: | OpenAIRE |
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