Stereochemical course of cobalamin-dependent radical SAM methylation by TokK and ThnK

Autor:	Michael S. Lichstrahl, Craig A. Townsend, Erica K. Sinner
Rok vydání:	2022
Předmět:	Chemistry (miscellaneous) Biochemistry Genetics and Molecular Biology (miscellaneous) Molecular Biology Biochemistry
Zdroj:	RSC Chemical Biology. 3:1028-1034
ISSN:	2633-0679
DOI:	10.1039/d2cb00113f
Popis:	Complex carbapenems are important clinical antibiotics for difficult-to-treat infections. An essential step in the biosyntheses of these natural products is stereospecific methylation at C6 and subsequent alkylations by cobalamin-dependent radical SAM methylases such as TokK and ThnK. We have prepared isotopically labeled substrates in a stereospecific manner and found that both homologous enzymes selectively abstract the 6-pro
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8240300f3e04829a206cdfeacc8f7da1 https://doi.org/10.1039/d2cb00113f Zobrazit plný text záznamu