Patterning of Peptide Nucleic Acids Using Reactive Microcontact Printing
| Autor: | Jurriaan Huskens, Vinod Subramaniam, Stefano Sforza, Geertruida A. Posthuma-Trumpie, Aart van Amerongen, Rosangela Marchelli, Roberto Corradini, Tullia Tedeschi, Pascal Jonkheijm, David N. Reinhoudt, Dorothee Wasserberg, Alessandro Calabretta |
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| Přispěvatelé: | Executive board Vrije Universiteit, Molecular Nanofabrication, Faculty of Science and Technology, Nanobiophysics |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Peptide Nucleic Acids
METIS-282159 Peptide Nanotechnology stereogenic centers Research Support Fluorescence handedness chemistry.chemical_compound Immune Regulation [NCMLS 2] Microscopy Electrochemistry Fluorescence microscope Journal Article General Materials Science Non-U.S. Gov't hybridization microarrays Spectroscopy pna chemistry.chemical_classification dna biosensors Polydimethylsiloxane Oligonucleotide Research Support Non-U.S. Gov't nanoparticle PDMS stamp Surfaces and Interfaces Condensed Matter Physics proteins IR-76903 BBP Bioconversion chemistry Microscopy Fluorescence Microcontact printing immobilization Nucleic acid recognition Wageningen Livestock Research |
| Zdroj: | Langmuir, 27, 1536-42 Langmuir, 27, 4, pp. 1536-42 Langmuir, 27(4), 1536-42. American Chemical Society Calabretta, A, Wasserberg, D, Posthuma-Trumpie, G A, Subramaniam, V, van Amerongen, A, Corradini, R, Tedeschi, T, Sforza, S, Reinhoudt, D N, Marchelli, R, Huskens, J & Jonkheijm, P 2011, ' Patterning of peptide nucleic acids using reactive microcontact printing ', Langmuir, vol. 27, no. 4, pp. 1536-42 . https://doi.org/10.1021/la102756k Langmuir, 27(4), 1536-1542 Langmuir 27 (2011) 4 Langmuir, 27(4), 1536-1542. American Chemical Society |
| ISSN: | 0743-7463 |
| DOI: | 10.1021/la102756k |
| Popis: | Item does not contain fulltext PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides. |
| Databáze: | OpenAIRE |
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