Detection of sulfur mustard adducts in human callus by phage antibodies
| Autor: | Govert P. van der Schans, Floris J. Bikker, A. Fidder, Roos H. Mars-Groenendijk, Daan Noort |
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| Rok vydání: | 2007 |
| Předmět: |
Phage display
viruses Biochemistry Bacteriophage chemistry.chemical_compound Drug Discovery Keratin Protein purification Mustard Gas Humans Genomic library Bacteriophages DNA Primers Pharmacology chemistry.chemical_classification Immunodiagnostics integumentary system biology Base Sequence Organic Chemistry Antibodies Monoclonal Sulfur mustard biology.organism_classification Molecular biology chemistry biology.protein Molecular Medicine Antibody |
| Zdroj: | Chemical biologydrug design. 69(5) |
| ISSN: | 1747-0277 |
| Popis: | As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time-consuming hybridoma technique. The Tomlinson I and J phage libraries were used to select phage antibodies exhibiting affinity for sulfur mustard adducts on keratins, isolated from human callus. Two kinds of phage antibodies were obtained: antibodies recognizing keratin and antibodies recognizing keratin which was exposed to sulfur mustard. These phage antibodies retained activity after repeated culturing and culturing in larger volumes. For the first time antibody phage display was successfully applied for immunodiagnostics of a chemical warfare agent. © 2007 The Authors. |
| Databáze: | OpenAIRE |
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