A Concise Review of Current In Vitro Chemical and Cell-Based Antioxidant Assay Methods
| Autor: | Roghayeh Amini Sarteshnizi, Ifeanyi D. Nwachukwu, Rotimi E. Aluko, Chibuike C. Udenigwe |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Radical Cells Pharmaceutical Science Organic chemistry free radicals Review 01 natural sciences Antioxidants Analytical Chemistry Lipid peroxidation chemistry.chemical_compound 0404 agricultural biotechnology QD241-441 Lipid oxidation Drug Discovery medicine Humans Physical and Theoretical Chemistry antioxidant assays chemistry.chemical_classification reactive oxygen species radical scavenging Reactive oxygen species cell culture Chemistry Superoxide 010401 analytical chemistry Cell Membrane lipid peroxidation 04 agricultural and veterinary sciences 040401 food science 0104 chemical sciences 3. Good health Biochemistry Chemistry (miscellaneous) Molecular Medicine Biological Assay Cell culture assays |
| Zdroj: | Molecules Molecules, Vol 26, Iss 4865, p 4865 (2021) |
| ISSN: | 1420-3049 |
| Popis: | Antioxidants remain interesting molecules of choice for suppression of the toxic effects of free radicals in foods and human systems. The current practice involves the use of mainly synthetic molecules as potent antioxidant agents. However, due to the potential negative impact on human health, there is an intensive effort within the research community to develop natural alternatives with similar antioxidant efficacy but without the negative side effects of synthetic molecules. Still, the successful development of new molecules depends on the use of reliable chemical or cell culture assays to screen antioxidant properties. Chemical antioxidant assays include the determination of scavenging ability against free radicals such as DPPH, superoxide anion radicals, hydroxyl radicals, hydrogen peroxide, and nitric oxide. Other antioxidant tests include the ability of compounds to bind and sequester prooxidant metal cations, reduce ferric iron, and attenuate the rate of lipid oxidation. Ex vivo tests utilize cell cultures to confirm entry of the molecules into cells and the ability to quench synthetic intracellular free radicals or to stimulate the increased biosynthesis of endogenous antioxidants. In order to assist researchers in their choice of antioxidant evaluation methods, this review presents background scientific information on some of the most commonly used antioxidant assays with a comparative discussion of the relevance of published literature data to food science and human nutrition applications. |
| Databáze: | OpenAIRE |
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