Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo
| Autor: | Lynn Harrison, Kanchanjunga Prasai, Kelly Tatchell, Lucy C. Robinson |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA Saccharomyces cerevisiae Proteins DNA Repair genetic processes Saccharomyces cerevisiae Biology DNA Mitochondrial Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Homologous chromosome DNA Breaks Double-Stranded DNA Fungal Molecular Biology fungi Cell Biology biology.organism_classification Molecular biology Yeast Double Strand Break Repair In vitro DNA-Binding Proteins enzymes and coenzymes (carbohydrates) 030104 developmental biology chemistry 030220 oncology & carcinogenesis health occupations Molecular Medicine biological phenomena cell phenomena and immunity DNA Transcription Factors |
| Zdroj: | Mitochondrion. 42:23-32 |
| ISSN: | 1567-7249 |
| DOI: | 10.1016/j.mito.2017.10.005 |
| Popis: | Mitochondrial DNA (mtDNA) double-strand break (DSB) repair is essential for maintaining mtDNA integrity, but little is known about the proteins involved in mtDNA DSB repair. Here, we utilize Saccharomyces cerevisiae as a eukaryotic model to identify proteins involved in mtDNA DSB repair. We show that Mhr1, a protein known to possess homologous DNA pairing activity in vitro, binds to mtDNA DSBs in vivo, indicating its involvement in mtDNA DSB repair. Our data also indicate that Yku80, a protein previously implicated in mtDNA DSB repair, does not compete with Mhr1 for binding to mtDNA DSBs. In fact, C-terminally tagged Yku80 could not be detected in yeast mitochondrial extracts. Therefore, we conclude that Mhr1, but not Yku80, is a potential mtDNA DSB repair factor in yeast. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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