A novel method of modifying immune responses by vaccination with lipiodol-siRNA mixtures
| Autor: | Zaohui Zhong, Hamid Izadi, Thomas E. Ichim, Neil H. Riordan, Li Yijian, E K Oleinik, Igor A. Popov, Salman Sher |
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| Rok vydání: | 2006 |
| Předmět: |
biology
business.industry Research medicine.medical_treatment lcsh:R lcsh:Medicine General Medicine Dendritic cell General Biochemistry Genetics and Molecular Biology Cell therapy Ovalbumin Immune system Antigen In vivo Immunology Cancer research biology.protein medicine Surgery business Adjuvant Ex vivo |
| Zdroj: | Journal of Translational Medicine, Vol 4, Iss 1, p 2 (2006) Surgery Publications Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Popis: | The dendritic cell (DC) possesses the ability to stimulate both T helper 1 (Th1) and Th2 responses depending on activation stimuli. Although it is known that chemically or genetically modified DC can be used therapeutically to steer immune responses towards either Th1 or Th2, cellular therapy with ex vivo manipulated DC is clinically difficult. Here we demonstrate a novel method of switching immune responses from Th1 to Th2 through in vivo immune modulation by administration of siRNA. We demonstrate that siRNA targeting of the IL-12p35 gene leads to a Th2 bias in vitro through an IL-10 dependent mechanism. In vivo administration of siRNA admixed with the oil-based contrast agent lipiodol in the presence of antigen and adjuvant induced a deviation in recall response to reduced production of IFN-γ and augmented IL-4 response using either KLH or ovalbumin. This simple method of in vivo modification of immune response possesses therapeutic potential in Th1-mediated diseases such as multiple sclerosis and autoimmune diabetes. |
| Databáze: | OpenAIRE |
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