Propylthiouracil-induced congenital hypothyroidism upregulates vimentin phosphorylation and depletes antioxidant defenses in immature rat testis
| Autor: | Danilo Wilhelm Filho, Kátia Padilha Barreto, Ariane Zamoner, Viviane Mara Woehl, Fátima Theresinha Costa Rodrigues Guma, Fátima Regina Mena Barreto Silva, Fabíola Sell, Regina Pessoa-Pureur |
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| Rok vydání: | 2008 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Vimentin medicine.disease_cause p38 Mitogen-Activated Protein Kinases Antioxidants Lipid peroxidation chemistry.chemical_compound Endocrinology Oxygen Consumption Internal medicine Testis medicine Congenital Hypothyroidism Animals Phosphorylation Rats Wistar Protein kinase A Cytoskeleton Intermediate filament Molecular Biology chemistry.chemical_classification Reactive oxygen species Sertoli Cells biology Seminiferous Tubules Sertoli cell Mitochondria Rats medicine.anatomical_structure chemistry Propylthiouracil biology.protein Lipid Peroxidation Reactive Oxygen Species Oxidative stress |
| Zdroj: | Journal of molecular endocrinology. 40(3) |
| ISSN: | 1479-6813 |
| Popis: | Congenital hypothyroidism was induced in rats by adding 0.05% 6-propyl-2-thiouracil in the drinking water from day 9 of gestation, and continually up to postnatal day 15. Structural alterations observed by light microscopy of seminiferous tubules and by transmission electron microscopy of Sertoli cells of treated animals were consistent with hypothyroid condition. Hypothyroidism was also associated with high phospho-p38 mitogen-activated protein kinase and decreased phospho-extracellular signal-regulated kinase 1/2 levels. Furthermore, the phosphorylation and the immunoreactivity of cytoskeletal-associated vimentin were increased without altering vimentin expression, suggesting an accumulation of insoluble and phosphorylated vimentin. These alterations in intermediate filament dynamics could result in loss of Sertoli cell cytoskeletal integrity and be somewhat related to the deleterious effects of hypothyroidism in testis. In addition, the mitochondrial alterations observed could also be related to defective cytoskeletal dynamics implying in cell damage. Moreover, we observed decreased oxygen consumption and unaltered lipid peroxidation in hypothyroid testis. However, we demonstrated decreased enzymatic and non-enzymatic antioxidant defenses, supporting an increased mitochondrial reactive oxygen species (ROS) generation, contributing to biochemical changes in hypothyroid testis. In addition, the changes in the testis histoarchitecture could be ascribed to cytoskeletal alterations, decreased antioxidant defenses, and increased ROS generation, leading to oxidative stress in the organ. |
| Databáze: | OpenAIRE |
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