MicroRNA-145 is a potential prognostic factor of scirrhous type gastric cancer
| Autor: | Kazuhiro Sentani, Keisuke Goto, Kazuyoshi Yanagihara, Masakazu Yashiro, Naohide Oue, Shunsuke Shinmei, Kosei Hirakawa, Kyohei Yasuno, Hiroko Tagawa, Naoya Sakamoto, Yutaka Naito, Htoo Zarni Oo, Wataru Yasui |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Adenocarcinoma Scirrhous Cell Biology Real-Time Polymerase Chain Reaction Stomach Neoplasms Cell Line Tumor microRNA medicine Biomarkers Tumor Humans Neoplasm Staging Oncogene Microarray analysis techniques Cancer General Medicine Cell cycle Middle Aged medicine.disease Prognosis Molecular medicine Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure Oncology Case-Control Studies Cancer cell Female Neoplasm Grading |
| Zdroj: | Oncology reports. 32(4) |
| ISSN: | 1791-2431 |
| Popis: | Gastric cancer (GC) is one of the most common malignancies worldwide. In particular, scirrhous type GC is highly metastatic and is characterized clinically by rapid disease progression and poor prognosis. MicroRNAs (miRNAs) play crucial roles in cancer development and progression. We previously demonstrated by microarray analysis that microRNA-145 (miR-145) is one of the more highly expressed miRNAs in scirrhous type GC vs. non-scirrhous types of GC. In the present study, we investigated the role of miR-145 in scirrhous type GC. The expression levels of miR-145 assessed by quantitative RT-PCR were higher in scirrhous type GC tissue samples than in non-scirrhous type GC and corresponding normal tissues. GC patients with high miR-145 expression were at a more advanced tumor stage (P=0.0156) and had more scirrhous type histology (P=0.0054) than those with low miR-145 expression. Furthermore, miR-145 expression was significantly associated with poor prognosis in GC patients (P=0.0438). miR-145 expression was localized in stromal fibroblasts of scirrhous type GC but not in cancer cells. miR-145 was induced by treatment by transforming growth factor-β, and it enhanced the expression of α-smooth muscle actin, a marker of myofibroblasts, in both normal gastric fibroblasts and cancer-associated fibroblasts. These data suggest that miR-145 may contribute to the progression of scirrhous type GC by regulating activation of peri-tumoral fibroblasts. |
| Databáze: | OpenAIRE |
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