Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers
| Autor: | Christina Stefani, Alfredo de la Escosura-Muñiz, Arben Merkoçi, Daniel Quesada-González, Neus Domingo, Israel González, Pere Mutjé |
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| Přispěvatelé: | Agencia Estatal de Investigación (España), Universidad Autónoma de Barcelona, Generalitat de Catalunya, Ministerio de Economía, Industria y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Economía y Competitividad (España), European Commission |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Scanning electron microscope Biomedical Engineering Biophysics Nanofibers Metal Nanoparticles Nanoparticle Biosensing Techniques 02 engineering and technology STRIPS 01 natural sciences law.invention chemistry.chemical_compound law Electrochemistry Humans Gold nanoparticles Cellulose Reagent Strips Lateral flow business.industry 010401 analytical chemistry Collodion Equipment Design General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Membrane Biosensors chemistry Colloidal gold Immunoglobulin G Nanofiber Optoelectronics Gold 0210 nano-technology business Antibodies Immobilized Biosensor Nitrocellulose Biotechnology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Recercat. Dipósit de la Recerca de Catalunya Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Popis: | Lateral flow paper-based biosensors merge as powerful tools in point-of-care diagnostics since they are cheap, portable, robust, selective, fast and easy to use. However, the sensitivity of this type of biosensors is not always as high as required, often not permitting a clear quantification. To improve the colorimetric response of standard lateral flow strips (LFs), we have applied a new enhancement strategy that increases the sensitivity of LFs based on the use of cellulose nanofibers (CNF). CNF penetrate inside the pores of LFs nitrocellulose paper, compacting the pore size only in the test line, particularly near the surface of the strip. This modification retains the bioreceptors (antibodies) close to the surface of the strips, and thus further increasing the density of selectively attached gold nanoparticles (AuNPs) in the top part of the membrane, in the test line area, only when the sample is positive. This effect boosts in average a 36.6% the sensitivity of the LFs. The optical measurements of the LFs were carried out with a mobile phone camera whose imaging resolution was improved by attaching microscopic lens on the camera objective. The characterization of CNF into paper and their effect was analyzed using atomic force microscope (AFM) and scanning electron microscope (SEM) imaging techniques. The ICN2 is funded by the CERCA Programme / Generalitat de Catalunya. The ICN2 is supported by the Severo Ochoa program of the Spanish Ministry of Economy, Industry and Competitiveness (MINECO, grant No. SEV-2017-0706). Financial support was obtained under MAT2017-87202-P, funded by Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional, and MINECO project FIS2015-73932-JIN. This work was partially funded by 2017-SGR-579 project from the Generalitat de Catalunya. D.Q.G. acknowledges Universitat Autònoma de Barcelona for the possibility of carrying out this work inside the framework of its PhD Programme in Chemistry. Ch.S. thanks the BIST for a preBIST Grant (No. 754558). |
| Databáze: | OpenAIRE |
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