A synthetic metabolic network for physicochemical homeostasis
| Autor: | Bauke F Gaastra, Wojciech M. Smigiel, Jacopo Frallicciardi, Shubham Singh, Bert Poolman, Tjeerd Pols, Hendrik R Sikkema |
|---|---|
| Přispěvatelé: | Enzymology |
| Rok vydání: | 2019 |
| Předmět: |
Ornithine
Hydrolases Cell volume General Physics and Astronomy Metabolic network 02 engineering and technology PATHWAY 0302 clinical medicine Adenosine Triphosphate lcsh:Science 0303 health sciences LACTIS Multidisciplinary synthetic cell MEMBRANE-PROTEINS Chemistry ARTIFICIAL CELL 021001 nanoscience & nanotechnology Transmembrane protein Enzymes Lactococcus lactis physicochemical homeostasis Osmolyte 0210 nano-technology Metabolic Networks and Pathways EXPRESSION Science Arginine Article out-of-equilibrium chemistry General Biochemistry Genetics and Molecular Biology 03 medical and health sciences metabolic network Atp production Ornithine Carbamoyltransferase 030304 developmental biology Metabolic energy Artificial cell DNA General Chemistry Phosphotransferases (Carboxyl Group Acceptor) TRANSPORT ATP RECONSTITUTION Biophysics Citrulline lcsh:Q Artificial Cells Carrier Proteins Energy Metabolism 030217 neurology & neurosurgery SYSTEM Homeostasis |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019) Nature Communications Nature Communications, 10(1):4239. Nature Publishing Group |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-12287-2 |
| Popis: | One of the grand challenges in chemistry is the construction of functional out-of-equilibrium networks, which are typical of living cells. Building such a system from molecular components requires control over the formation and degradation of the interacting chemicals and homeostasis of the internal physical-chemical conditions. The provision and consumption of ATP lies at the heart of this challenge. Here we report the in vitro construction of a pathway in vesicles for sustained ATP production that is maintained away from equilibrium by control of energy dissipation. We maintain a constant level of ATP with varying load on the system. The pathway enables us to control the transmembrane fluxes of osmolytes and to demonstrate basic physicochemical homeostasis. Our work demonstrates metabolic energy conservation and cell volume regulatory mechanisms in a cell-like system at a level of complexity minimally needed for life. Functional out-of-equilibrium networks are typical of living cells. Here the authors report the construction of a sustained ATP production system in vesicles with controlled energy dissipation and physicochemical homeostasis. |
| Databáze: | OpenAIRE |
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