High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
| Autor: | Cristina Isabel Amador, Rune Overlund Stannius, Mette Burmølle, Henriette Lyng Røder |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Xanthomonas High-throughput screening Microbacterium High Throughput Assay Microbiology Fluorescence Imaging chemistry.chemical_compound Mixed species Reproductubility Crystal violet Molecular Biology Reproducibility Chromatography Cytation5 Bacteria Chemistry Pseudomonas putida Biofilm Optical Imaging biochemical phenomena metabolism and nutrition High-Throughput Screening Assays Stenotrophomonas Biofilms Gentian Violet Paenibacillus Fluorescence labelling Plate reader High throughput assay |
| Zdroj: | Amador, C I, Stannius, R O, Røder, H L & Burmølle, M 2021, ' High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging ', Journal of Microbiological Methods, vol. 190, 106343 . https://doi.org/10.1016/j.mimet.2021.106343 |
| DOI: | 10.1016/j.mimet.2021.106343 |
| Popis: | The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm. |
| Databáze: | OpenAIRE |
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