Tubulin homolog TubZ in a phage-encoded partition system
| Autor: | José Manuel Andreu, Yoshihiko Sakaguchi, María A. Oliva, Antonio J. Martin-Galiano |
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| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular Cell division Centromere Molecular Sequence Data Biophysics Bacteriophage chemistry.chemical_compound Plasmid Bacterial Proteins Tubulin Clostridium botulinum Cluster Analysis Bacteriophages Amino Acid Sequence Cloning Molecular Gene Peptide sequence Phylogeny Cytokinesis Genetics Multidisciplinary Base Sequence biology Computational Biology Sequence Analysis DNA Biological Sciences biology.organism_classification Cell biology chemistry Multiprotein Complexes biology.protein Crystallization DNA Plasmids |
| Zdroj: | Proceedings of the National Academy of Sciences. 109:7711-7716 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 Å structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region ( tubS ), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R- S complex interaction. |
| Databáze: | OpenAIRE |
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