Molecular Characterization of Acquired Enrofloxacin Resistance in Mycoplasma synoviae Field Isolates
| Autor: | Inna Lysnyansky, F. Gobbo, Irena Gerchman, Inna Mikula, S. Catania, S. Levisohn |
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| Rok vydání: | 2013 |
| Předmět: |
DNA Topoisomerase IV
Genotype medicine.drug_class Sequence analysis animal diseases Molecular Sequence Data Microbial Sensitivity Tests Drug resistance Mycoplasma synoviae Quinolones Biology DNA gyrase Microbiology Bacterial Proteins Mechanisms of Resistance Lectins Drug Resistance Bacterial medicine Enrofloxacin Mycoplasma Infections Pharmacology (medical) Amino Acid Sequence Israel Pharmacology Base Sequence Broth microdilution Sequence Analysis DNA biochemical phenomena metabolism and nutrition Quinolone Anti-Bacterial Agents Europe Molecular Typing Infectious Diseases Amino Acid Substitution DNA Gyrase Fluoroquinolones medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 57:3072-3077 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00203-13 |
| Popis: | The in vitro activity of enrofloxacin against 73 Mycoplasma synoviae field strains isolated in Israel and Europe was determined by broth microdilution. Decreased susceptibility to enrofloxacin was identified in 59% of strains, with the MICs ranging from 1 to >16 μg/ml. The estimated MIC 50 and MIC 90 values for enrofloxacin were 2 and 8 μg/ml, respectively. Moreover, this study showed that 92% of recent Israeli field isolates (2009 to 2011) of M. synoviae have MICs of ≥2 μg/ml to enrofloxacin. Comparison of the quinolone resistance-determining regions (QRDRs) in M. synoviae isolates revealed a clear correlation between the presence of one of the amino acid substitutions Asp79-Asn, Thr80-Ala/Ile, Ser81-Pro, and Asp84-Asn/Tyr/His of the ParC QRDR and decreased susceptibility to enrofloxacin (MIC, ≥1 μg/ml). Amino acid substitutions at positions GyrA 87, GyrB 401/402, and ParE 420/454 were also identified, but there was no clear-cut correlation with susceptibility to enrofloxacin. Comparison of vlhA molecular profiles revealed the presence of 9 different genotypes in the Israeli M. synoviae field isolates and 10 genotypes in the European isolates; only one vlhA genotype (type 4) was identified in both cohorts. Based on results of vlhA molecular typing, several mechanisms for emergence and dissemination of Israeli enrofloxacin-resistant M. synoviae isolates are suggested. |
| Databáze: | OpenAIRE |
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