Recombinant Filamentous Bacteriophages Encapsulated in Biodegradable Polymeric Microparticles for Stimulation of Innate and Adaptive Immune Responses
| Autor: | Piergiuseppe De Berardinis, Concetta Di Natale, Rossella Sartorius, Paolo A. Netti, Raffaele Vecchione, Rezvan Jamaledin |
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| Přispěvatelé: | Jamaledin, R., Sartorius, R., Di Natale, C., Vecchione, R., De Berardinis, P., Netti, P. A. |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 02 engineering and technology medicine.disease_cause Microbiology Epitope Article law.invention Bacteriophage 03 medical and health sciences chemistry.chemical_compound Immune system law Virology medicine sustained-release Escherichia coli lcsh:QH301-705.5 microparticles biology Chemistry Immunogenicity 021001 nanoscience & nanotechnology biology.organism_classification vaccine delivery immunity Cell biology PLGA 030104 developmental biology Capsid lcsh:Biology (General) Microparticle bacteriophage display Recombinant DNA 0210 nano-technology |
| Zdroj: | Microorganisms Volume 8 Issue 5 Microorganisms, Vol 8, Iss 650, p 650 (2020) Microorganisms 8 (2020). doi:10.3390/microorganisms8050650 info:cnr-pdr/source/autori:Jamaledin R.; Sartorius R.; Di Natale C.; Vecchione R.; De Berardinis P.; Netti P.A./titolo:Recombinant filamentous bacteriophages encapsulated in biodegradable polymeric microparticles for stimulation of innate and adaptive immune responses/doi:10.3390%2Fmicroorganisms8050650/rivista:Microorganisms/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:8 |
| ISSN: | 2076-2607 |
| Popis: | Escherichia coli filamentous bacteriophages (M13, f1, or fd) have attracted tremendous attention from vaccinologists as a promising immunogenic carrier and vaccine delivery vehicle with vast possible applications in the development of vaccines. The use of fd bacteriophage as an antigen delivery system is based on a modification of bacteriophage display technology. In particular, it is designed to express multiple copies of exogenous peptides (or polypeptides) covalently linked to viral capsid proteins. This study for the first time proposes the use of microparticles (MPs) made of poly (lactic-co-glycolic acid)(PLGA) to encapsulate fd bacteriophage. Bacteriophage&ndash PLGA MPs were synthesized by a water in oil in water (w1/o/w2) emulsion technique, and their morphological properties were analyzed by confocal and scanning electron microscopy (SEM). Moreover, phage integrity, encapsulation efficiency, and release were investigated. Using recombinant bacteriophages expressing the ovalbumin (OVA) antigenic determinant, we demonstrated the immunogenicity of the encapsulated bacteriophage after being released by MPs. Our results reveal that encapsulated bacteriophages are stable and retain their immunogenic properties. Bacteriophage-encapsulated PLGA microparticles may thus represent an important tool for the development of different bacteriophage-based vaccine platforms. |
| Databáze: | OpenAIRE |
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