β1 integrin is necessary for ureteric bud branching morphogenesis and maintenance of collecting duct structural integrity
| Autor: | Ambra Pozzi, Raoul D. Nelson, Manakan B. Srichai, Dong-Hua Yang, Glenda Mernaugh, Dale R. Abrahamson, Raymond C. Harris, Juan M. Iturregui, Donald E. Kohan, Xi Zhang, Roy Zent, Leslie Gewin, Peter D. Yurchenco, Reinhard Fässler |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cell signaling
Integrin Morphogenesis Gestational Age Mice Transgenic Fibroblast growth factor Cell Line Mice Organ Culture Techniques Cell Movement Cell Adhesion Glial cell line-derived neurotrophic factor Animals Kidney Tubules Collecting Growth Substances Cell adhesion Molecular Biology Cell Proliferation Mice Knockout biology Integrin beta1 Gene Expression Regulation Developmental Cell biology Ureteric bud biology.protein Development and Disease Ureter Signal transduction Gene Deletion Signal Transduction Developmental Biology |
| Zdroj: | Development. 136:3357-3366 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.036269 |
| Popis: | The kidney collecting system develops from branching morphogenesis of the ureteric bud (UB). This process requires signaling by growth factors such as glial cell line derived neurotrophic factor (GDNF) and fibroblast growth factors (FGFs) as well as cell extracellular matrix interactions mediated by integrins. The importance of integrin signaling in UB development was investigated by deleting integrin β1 at initiation (E10.5) and late(E18.5) stages of development. Deletion at E10.5 resulted in a severe branching morphogenesis phenotype. Deletion at E18.5 did not alter renal development but predisposed the collecting system to severe injury following ureteric obstruction. β1 integrin was required for renal tubular epithelial cells to mediate GDNF- and FGF-dependent signaling despite normal receptor localization and activation in vitro. Aberrations in the same signaling molecules were present in the β1-null UBs in vivo. Thus β1 integrins can regulate organ branching morphogenesis during development by mediating growth-factor-dependent signaling in addition to their well-defined role as adhesion receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|