Detection of Wilms' tumor antigen--specific CTL in tumor-draining lymph nodes of patients with early breast cancer
| Autor: | Hans J. Stauss, Suzanne Parry, Dimitri Hadjiminas, Shao-An Xue, R. Charles Coombes, Roberto Dina, Jonathan Waxman, Jaspal Kaeda, Ilaria Bellantuono, Yasmeen Ghani, Vourneen Healy, Angelika Holler, Roopinder Gillmore |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
medicine.medical_treatment Major histocompatibility complex Breast cancer Antigen Antigens Neoplasm Cell Line Tumor HLA-A2 Antigen Cytotoxic T cell Medicine Humans WT1 Proteins Lymph node Aged biology business.industry Reverse Transcriptase Polymerase Chain Reaction fungi Immunotherapy Middle Aged medicine.disease Cytotoxicity Tests Immunologic Flow Cytometry Tumor antigen CTL medicine.anatomical_structure Oncology Immunology biology.protein Female Lymph Nodes business T-Lymphocytes Cytotoxic |
| Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 12(1) |
| ISSN: | 1078-0432 |
| Popis: | Purpose: The Wilms' tumor antigen (WT1) is overexpressed in ∼90% of breast tumors and, thus, is a potential target antigen for the immunotherapy of breast cancer. We have tested the working hypotheses that WT1 can be immunogenic in patients with breast cancer and can stimulate CTL of sufficient avidity to kill tumor cells.Experimental Design: Paired tumor-draining lymph node and peripheral blood samples were analyzed from five HLA-A2-positive patients with stage I/II breast cancer. Fluorescent HLA-A*0201/WT1 tetramers were used to quantify WT1-specific CTL and the functional capacity of the CTL was assessed using cytotoxicity assays and intracellular cytokine staining.Results: WT1 tetramer–binding T cells expanded from all lymph node samples but none of the corresponding peripheral blood samples. Functional assays were carried out on T cells from the patient who had yielded the highest frequency of HLA-A*0201/WT1 tetramer–positive cells. The cytotoxicity assays showed WT1 peptide–specific killing activity of the CTL, whereas intracellular cytokine staining confirmed that the tetramer–positive T cells produced IFN-γ after stimulation with WT1 peptide. These WT1-specific T cells killed HLA-A2-positive breast cancer cell lines treated with IFN-γ but no killing was observed with untreated tumor cells.Conclusions: These results show that WT1-specific CTL can be expanded from the tumor-draining lymph nodes of breast cancer patients and that they can display peptide-specific effector function. However, the CTL only killed IFN-γ-treated tumor targets expressing high levels of HLA-A2 and not tumor cells with low HLA expression. This suggests that induction of autologous WT1-specific CTL may offer only limited tumor protection and that strategies that allow a high level of peptide/MHC complex presentation and/or improve CTL avidity may be required. |
| Databáze: | OpenAIRE |
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