Desensitization of beta-adrenergic receptors in lung injury induced by 2-chloroethyl ethyl sulfide, a mustard analog
| Autor: | Syeda M. Kabir, Salil K. Das, Veera Rajaratnam, Milton G. Smith, Shyamali Mukherjee |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Cholera Toxin IBMX Health Toxicology and Mutagenesis Guinea Pigs Intracellular Space Stimulation Lung injury Toxicology medicine.disease_cause Biochemistry Article chemistry.chemical_compound Internal medicine Pulmonary fibrosis Mustard Gas Receptors Adrenergic beta medicine Cyclic AMP Animals Molecular Biology Lung Membranes Cholera toxin General Medicine Lung Injury medicine.disease medicine.anatomical_structure Endocrinology chemistry Gene Expression Regulation Dihydroalprenolol Molecular Medicine Cyclase activity |
| Zdroj: | Journal of biochemical and molecular toxicology. 23(1) |
| ISSN: | 1099-0461 |
| Popis: | 2-Choloroethyl Ethyl Sulfide (CEES) exposure causes inflammatory lung diseases, including acute respiratory distress syndrome (ARDS) and pulmonary fibrosis. This may be associated with oxidative stress, which has been implicated in the desensitization of beta-adrenergic receptors (β-ARs). The objective of this study was to investigate whether lung injury induced by intratracheal CEES exposure (2 mg/kg body weight) causes desensitization of β-ARs. The animals were sacrificed after 7 days and lungs were removed. Lung injury was established by measuring the leakage of iodinated-bovine serum albumin ([125I]-BSA) into lung tissue. Receptor-binding characteristics were determined by measuring the binding of [3H] dihydroalprenolol ([3H] DHA) (0.5–24 nM) to membrane fraction in the presence and absence of DLDL-propranolol (10 μ M). Both high- and low-affinity β-ARs were identified in the lung. Binding capacity was significantly higher in low-affinity site in both control and experimental groups. Although CEES exposure did not change KD and Bmax at the high-affinity site, it significantly decreased both KD and Bmax at low affinity sites. A 20% decrease in β2-AR mRNA level and a 60% decrease in membrane protein levels were observed in the experimental group. Furthermore, there was significantly less stimulation of adenylate cyclase activity by both cholera toxin and isoproterenol in the experimental group in comparison to the control group. Treatment of lungs with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterase (PDE) could not abolish the difference between the control group and the experimental group on the stimulation of the adenylate cyclase activity. Thus, our study indicates that CEES-induced lung injury is associated with desensitization of β2-AR. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:59–70, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20265 |
| Databáze: | OpenAIRE |
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