Retraction: Detection, Characterization, and Spontaneous Differentiation In Vitro of Very Small Embryonic-Like Putative Stem Cells in Adult Mammalian Ovary
| Autor: | Vinita Daithankar, Seema Parte, Deepa Bhartiya, Kusum Zaveri, Jyoti Telang, Indira Hinduja, Vinita Salvi |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Homeobox protein NANOG Stage-Specific Embryonic Antigens endocrine system Cellular differentiation Cell Culture Techniques Biology Oogonial Stem Cells Oogenesis Original Research Reports medicine Animals Humans Cells Cultured Embryonic Stem Cells Cell Proliferation Sheep Ovary Cell Differentiation Haplorhini Cell Biology Hematology Middle Aged Embryonic stem cell Molecular biology Epithelium medicine.anatomical_structure Cell culture Oocytes Female Rabbits Menopause Stem cell Octamer Transcription Factor-3 Developmental Biology |
| Zdroj: | Stem Cells and Development. 20:1451-1464 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2010.0461 |
| Popis: | The present study was undertaken to detect, characterize, and study differentiation potential of stem cells in adult rabbit, sheep, monkey, and menopausal human ovarian surface epithelium (OSE). Two distinct populations of putative stem cells (PSCs) of variable size were detected in scraped OSE, one being smaller and other similar in size to the surrounding red blood cells in the scraped OSE. The smaller 1–3 μm very small embryonic-like PSCs were pluripotent in nature with nuclear Oct-4 and cell surface SSEA-4, whereas the bigger 4–7 μm cells with cytoplasmic localization of Oct-4 and minimal expression of SSEA-4 were possibly the tissue committed progenitor stem cells. Pluripotent gene transcripts of Oct-4, Oct-4A, Nanog, Sox-2, TERT, and Stat-3 in human and sheep OSE were detected by reverse transcriptase–polymerase chain reaction. The PSCs underwent spontaneous differentiation into oocyte-like structures, parthenote-like structures, embryoid body-like structures, cells with neuronal-like phenotype, and embryonic stem cell-like colonies, whereas the epithelial cells transformed into mesenchymal phenotype by epithelial–mesenchymal transition in 3 weeks of OSE culture. Germ cell markers like c-Kit, DAZL, GDF-9, VASA, and ZP4 were immuno-localized in oocyte-like structures. In conclusion, as opposed to the existing view of OSE being a bipotent source of oocytes and granulosa cells, mammalian ovaries harbor distinct very small embryonic-like PSCs and tissue committed progenitor stem cells population that have the potential to develop into oocyte-like structures in vitro, whereas mesenchymal fibroblasts appear to form supporting granulosa-like somatic cells. Research at the single-cell level, including complete gene expression profiling, is required to further confirm whether postnatal oogenesis is a conserved phenomenon in adult mammals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|