Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects
| Autor: | Rúbia Teodoro Stuepp, Andréa Gonçalves Trentin, Priscilla Barros Delben, Filipe Modolo, Michelle Tillmann Biz, Ricardo Castilho Garcez |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Pathology medicine.medical_specialty Histology Adolescent 0206 medical engineering H&E stain CD34 02 engineering and technology Mandible stomatognathic system Osteogenesis Dental pulp stem cells medicine Animals Humans CD90 Rats Wistar Bone regeneration Cells Cultured Dental Pulp Permanent teeth business.industry Regeneration (biology) Stem Cells 021001 nanoscience & nanotechnology 020601 biomedical engineering Mandibular Injuries Rats stomatognathic diseases Anatomy Stem cell 0210 nano-technology business Stem Cell Transplantation |
| Zdroj: | Cells, tissues, organs. 207(3-4) |
| ISSN: | 1422-6421 |
| Popis: | This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Before surgery procedures, the human dental pulp cells were characterized as dental pulp stem cells: fusiform morphology, plastic-adherent; expression of CD105, CD73, and CD90; lack of expression of CD45 and CD34, and differentiated into osteoblasts, adipocytes, and chondroblasts. The results indicated that within 7 days the control group presented a pronounced bone formation when compared with the treated group (p < 0.05). After 14 days, the treated group showed an increase in bone formation, but with no statistical difference among the groups (p > 0.05). In the final evaluated period there was no difference between the control group and the treated group (p > 0.05). There was a significant difference between 7 and 14 days (p < 0.05) and between 7 and 28 days (p < 0.05) in the treated group. In conclusion, there is no evidence that the use of hDPSCs in the conditions of this study could improve bone formation in non-critical-sized mandibular bone defects. |
| Databáze: | OpenAIRE |
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