Detection of polyomaviral DNA in clinical samples from immunocompromised patients: Correlation with clinical disease
| Autor: | Robert F. Miller, N S Brink, J D Fox, C. J. Perrons, Richard S. Tedder, Sebastian Lucas |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty viruses JC virus HIV Infections Simian virus 40 Biology medicine.disease_cause Polymerase Chain Reaction Virus law.invention Immunocompromised Host law medicine Humans Polymerase chain reaction Cerebrospinal Fluid Slow virus Progressive multifocal leukoencephalopathy Leukoencephalopathy Progressive Multifocal Brain virus diseases medicine.disease JC Virus Virology United Kingdom CD4 Lymphocyte Count BK virus Infectious Diseases BK Virus DNA Viral Viral disease Nested polymerase chain reaction |
| Zdroj: | Journal of Infection. 32:205-209 |
| ISSN: | 0163-4453 |
| DOI: | 10.1016/s0163-4453(96)80020-x |
| Popis: | Clinical samples from immunocompromised patients were screened for polyomaviral sequences by nested polymerase chain reaction (PCR) to evaluate the association of these viral infections with progressive multifocal leukoencephalopathy (PML). JC virus (JCV) DNA was detected in 19 of 23 CSF samples and all four brain samples from patients with PML. Neither BK virus (BKV) nor simian virus 40 (SV40) DNA were detected in these samples. No evidence was found to support the hypothesis that polyomaviral DNA is present in the central nervous system of immunosuppressed patients without PML (CSF n = 67, brain n = 19). JCV DNA was not detected in any peripheral blood sample included in this study. JCV DNA was detected in urine from three of eight patients with PML, but was also amplified from three of 29 urine samples from patients without PML, JCV, and not SV40 or BKV, was associated with PML in this study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect