Proteomic profile of pre-implantational ovine embryos produced in vivo
| Autor: | Fábio Roger Vasconcelos, Marcelo Bertolini, Mariana S. Castro, Carlos André Ornelas Ricart, Ivan Cunha Bustamante-Filho, Wagner Fontes, Aline Maria Araújo Martins, A. G. A. Viana, Arlindo A. Moura, Deisy J D Sanchez, Antônio C A Teles-Filho, Marcelo Valle de Sousa |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Proteome Biology MiRBase 03 medical and health sciences Endocrinology medicine Animals Blastocyst Gene Insemination Artificial Sheep Domestic 030304 developmental biology 0303 health sciences 0402 animal and dairy science Wnt signaling pathway Embryo 04 agricultural and veterinary sciences Cell cycle Embryo Mammalian 040201 dairy & animal science Embryonic stem cell Cell biology MicroRNAs medicine.anatomical_structure embryonic structures Animal Science and Zoology Female Biotechnology |
| Zdroj: | Reproduction in domestic animals = ZuchthygieneREFERENCES. 56(4) |
| ISSN: | 1439-0531 |
| Popis: | The present study was conducted to decipher the proteome of in vivo-produced pre-implantation ovine embryos. Ten locally adapted Morana Nova ewes received hormonal treatment and were inseminated 12 hr after ovulation. Six days later, 54 embryos (morula and blastocyst developmental state) were recovered from eight ewes and pooled to obtain sufficient protein for proteomic analysis. Extracted embryo proteins were analysed by LC-MS/MS, followed by identification based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, PepExplorer). Identified proteins were analysed for gene ontology terms, protein clusters and interactions. Genes associated with the ovine embryo proteome were screened for miRNA targets using data sets of TargetScan (http://www.targetscan.org) and mIRBase (http://www.mirbase.org) servers. There were 667 proteins identified in the ovine embryos. Biological processes of such proteins were mainly related to cellular process and regulation, and molecular functions, to binding and catalytic activity. Analysis of the embryo proteins revealed 49 enriched functional clusters, linked to energy metabolism (TCA cycle, pyruvate and glycolysis metabolism), zona pellucida (ZP), MAPK signalling pathway, tight junction, binding of sperm to ZP, translation, proteasome, cell cycle and calcium/phospholipid binding. Sixteen miRNAs were related to 25 pre-implantation ovine embryo genes, all conserved in human, bovine and ovine species. The interaction network generated by miRNet showed four key miRNAs (hsa-mir-106b-5p; hsa-mir-30-5p; hsa-mir-103a-5p and hsa-mir-106a-5p) with potential interactions with embryo-expressed genes. Functional analysis of the network indicated that miRNAs modulate genes related to cell cycle, regulation of stem cell and embryonic cell differentiation, among others. Retrieved miRNAs also modulate the expression of genes involved in cell signalling pathways, such as MAPK, Wnt, TGF-beta, p53 and Toll-like receptor. The current study describes the first major proteomic profile of 6-day-old ovine embryos produced in vivo, setting a comprehensive foundation for our understanding of embryo physiology in the ovine species. |
| Databáze: | OpenAIRE |
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