Neuroprotective effects of intravenous immunoglobulin are mediated through inhibition of complement activation and apoptosis in a rat model of sepsis
Autor: | Erdem Tüzün, Sema Bilgic Gazioglu, Perihan Ergin Özcan, Murat Giriş, Melike Küçükerden, Nadir Arican, Uğur Akcan, Günseli Orhun, Nurcan Orhan, Evren Şentürk, Canan Yilmaz, Figen Esen, Mehmet Kaya |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Immunoglobulins Apoptosis Inflammation Complement receptor Pharmacology Critical Care and Intensive Care Medicine Glial cell proliferation C5a receptor 03 medical and health sciences 0302 clinical medicine Sepsis medicine biology Glial fibrillary acidic protein business.industry Research Cecal ligation and puncture Complement activation Complement system 030104 developmental biology Integrin alpha M Gliosis Immunology biology.protein medicine.symptom business 030217 neurology & neurosurgery |
Zdroj: | Intensive Care Medicine Experimental |
ISSN: | 2197-425X |
Popis: | Background Intravenous (IV) immunoglobulin (Ig) treatment is known to alleviate behavioral deficits and increase survival in the experimentally induced model of sepsis. To delineate the mechanisms by which IVIg treatment prevents neuronal dysfunction, an array of immunological and apoptosis markers was investigated. Methods Sepsis was induced by cecal ligation perforation (CLP) in rats. The animals were divided into five groups: sham, control, CLP + saline, CLP + immunoglobulin G (IgG) (250 mg/kg, iv), and CLP + immunoglobulins enriched with immunoglobulin M (IgGAM) (250 mg/kg, iv). Blood and brain samples were taken in two sets of experiments to see the early (24 h) and late (10 days) effects of treatment. Total complement activity, complement 3 (C3), and soluble complement C5b-9 levels were measured in the sera of rats using ELISA-based methods. Cerebral complement, complement receptor, NF-κB, Bax, and Bcl-2 expressions were analyzed by western blot and/or RT-PCR methods. Immune cell infiltration and gliosis were examined by immunohistochemistry using CD3, CD4, CD8, CD11b, CD19, and glial fibrillary acidic protein antibodies. Apoptotic neuronal death was investigated by TUNEL staining. Results IVIgG and IgGAM administration significantly reduced systemic complement activity and cerebral C5a and C5a receptor expression. Likewise, both treatment methods reduced proapoptotic NF-κB and Bax expressions in the brain. IVIgG and IgGAM treatment induced considerable amelioration in glial cell proliferation and neuronal apoptosis which were increased in non-treated septic rats. Conclusions We suggest that IVIgG and IgGAM administration ameliorates neuronal dysfunction and behavioral deficits by reducing apoptotic cell death and glial cell proliferation. In both treatment methods, these beneficial effects might be mediated through reduction of anaphylatoxic C5a activity and subsequent inhibition of inflammation and apoptosis pathways. Electronic supplementary material The online version of this article (doi:10.1186/s40635-016-0114-1) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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