Performance of Platelin LS and dilute Russell's viper venom for the screening of lupus anticoagulant in patients with venous thromboembolism
| Autor: | Dayse Maria Lourenço, Maria Aparecida Eiko Noguti, Vânia Maris Morelli, Marinez Farana Matos, Raniely Casimiro da Silveira, Aline Amorim Martinez Ribeiro, Celso Arrais Rodrigues |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Dilute Russell's viper venom time Adolescent Viper Venoms Gastroenterology Internal medicine medicine Coagulopathy Humans Child Aged Aged 80 and over Lupus anticoagulant medicine.diagnostic_test business.industry Venous Thromboembolism Hematology General Medicine Middle Aged Mixing study medicine.disease Thrombosis Venous thrombosis Lupus Coagulation Inhibitor Anesthesia Prothrombin Time Female Partial Thromboplastin Time Reagent Kits Diagnostic business Venous thromboembolism circulatory and respiratory physiology Partial thromboplastin time |
| Zdroj: | Blood Coagulation & Fibrinolysis. 18:401-405 |
| ISSN: | 0957-5235 |
| DOI: | 10.1097/mbc.0b013e32814fcc50 |
| Popis: | Lupus anticoagulant is associated with thrombosis and pregnancy morbidity, and its detection is of major clinical importance. The nature and concentration of phospholipids strongly influence the sensitivity of activated partial thromboplastin time (aPTT) reagents to lupus anticoagulant. We investigated the ability of Platelin LS, an aPTT reagent, to screen lupus anticoagulant among 94 patients with venous thromboembolism by comparing its performance with the dilute Russell viper venom time (dRVVT). Twenty-four patients had an abnormal aPTT and dRVVT, whereas 37 only had a prolonged dRVVT. In users of oral anticoagulants (n = 56), the dRVVT prolonged more frequently than the aPTT (98.2 vs 39.3%, P < 0.0001). After the mixing study, seven patients maintained abnormal aPTT and dRVVT ratios, five of whom had prolonged mixture with both tests. The agreement in the mixing study between aPTT and dRVVT was substantial (kappa = 0.78, 95% confidence interval = 0.48-1.00). Except for one patient, the aPTT screened all cases that demonstrated phospholipid dependency of their inhibitor during the confirmatory procedure with the dRVVT. In conclusion, the aPTT using Platelin LS was highly associated with the presence of lupus anticoagulant detected by the dRVVT among patients with venous thromboembolism, and could be reliably employed as a screening assay for lupus anticoagulant. |
| Databáze: | OpenAIRE |
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