A large‐scale genomic association analysis identifies a fragment in Dt11 chromosome conferring cotton Verticillium wilt resistance

Autor:	Rongkang Shi, Yan Zhang, Zhikun Li, Zhengwen Sun, Bin Chen, Song Liu, Yanru Cui, Guiyin Zhang, Liqiang Wu, Ke Huifeng, Wang Guoning, Jun Yang, Xingfen Wang, Zhengwen Liu, Zhicheng Wang, Wu Jinhua, Zhiying Ma
Rok vydání:	2021
Předmět:	Quantitative Trait Loci Population Gossypium hirsutum Single-nucleotide polymorphism Plant Science Verticillium Biology Chromosomes Ascomycota Genotype Genetic variation GWAS Plant breeding Verticillium dahliae Allele education Dt11 chromosome Research Articles GhLecRKs gene cluster Disease Resistance Plant Diseases Genetics Gossypium education.field_of_study Verticillium wilt Genomics biology.organism_classification Plant Breeding Agronomy and Crop Science Research Article Biotechnology
Zdroj:	Plant Biotechnology Journal
ISSN:	1467-7652 1467-7644
DOI:	10.1111/pbi.13650
Popis:	Summary Verticillium wilt (VW) is a destructive disease that results in great losses in cotton yield and quality. Identifying genetic variation that enhances crop disease resistance is a primary objective in plant breeding. Here we reported a GWAS of cotton VW resistance in a natural‐variation population, challenged by different pathogenicity stains and different environments, and found 382 SNPs significantly associated with VW resistance. The associated signal repeatedly peaked in chromosome Dt11 (68 798 494‐69 212 808) containing 13 core elite alleles undescribed previously. The core SNPs can make the disease reaction type from susceptible to tolerant or resistant in accessions with alternate genotype compared to reference genotype. Of the genes associated with the Dt11 signal, 25 genes differentially expressed upon Verticillium dahliae stress, with 21 genes verified in VW resistance via gene knockdown and/or overexpression experiments. We firstly discovered that a gene cluster of L‐type lectin‐domain containing receptor kinase (GhLecRKs‐V.9) played an important role in VW resistance. These results proved that the associated Dt11 region was a major genetic locus responsible for VW resistance. The frequency of the core elite alleles (FEA) in modern varieties was significantly higher than the early/middle varieties (12.55% vs 4.29%), indicating that the FEA increased during artificial selection breeding. The current developmental resistant cultivars, JND23 and JND24, had fixed these core elite alleles during breeding without yield penalty. These findings unprecedentedly provided genomic variations and promising alleles for promoting cotton VW resistance improvement.
Databáze:	OpenAIRE
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