Serum levels of tumor necrosis factor-alpha, interleukin-1, and interferon-gamma in beta(o)-thalassemia/HbE and their clinical significance
| Autor: | S. Chinprasertsuk, F. Mawas, S. Visudhiphan, E Wiener, P. Pootrakul, U. Siripanyaphinyo, Wanchai Wanachiwanawin, Suthat Fucharoen, S.N. Wickramasinghe |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Anemia medicine.medical_treatment Immunology Enzyme-Linked Immunosorbent Assay Biology Interferon-gamma Reticulocyte Virology Internal medicine medicine Humans Interferon gamma Phagocytes Tumor Necrosis Factor-alpha Hemoglobin E beta-Thalassemia Interleukin Cell Biology Syndrome Middle Aged medicine.disease Hematologic Diseases Endocrinology Cytokine medicine.anatomical_structure Erythropoiesis Tumor necrosis factor alpha Female Hemoglobin medicine.drug Interleukin-1 |
| Zdroj: | Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 19(2) |
| ISSN: | 1079-9907 |
| Popis: | Serum levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1alpha (IL-1alpha), and interferon-gamma (IFN-gamma) were estimated by conventional ELISA kits in 60, 42, and 58 Thai patients, respectively, with beta(o)-thalassemia HbE and found to be above the normal range in 13%, 21%, and 33% of the patients, respectively. Using high-sensitivity ELISA systems, an additional 10 beta(o)-thal/HbE patients were compared with 9 controls for concentrations of circulating TNF-alpha and IL-1beta, and 9 and 5 patients, respectively, but only 1 and none of the controls, respectively, showed values above the normal ranges. In patients with abnormally high IFN-gamma levels, basal hemoglobin values were significantly lower than in those with normal levels of the cytokine (mean +/- SEM: 6.03+/-0.24 vs. 7.08+/-0.18, p < 0.05), although circulating concentrations of soluble transferrin receptors (sTrF) and absolute reticulocyte counts were similar in the two groups. Patients with raised or normal levels of TNF-alpha, IL-1alpha, or IL-1beta had similar basal hemoglobin values. In a phagocytosis assay, monocytes of patients with raised serum levels of IFN-gamma showed significantly more attached or ingested IgG-coated red cells than those of patients with normal concentrations of the cytokine (mean +/- SEM: 192+/-22 vs. 140+/-14 per 100 monocytes, p < 0.05). Moreover, in 3 of 4 of the former patients, the number of attached or ingested IgG-coated red cells per 100 monocytes was above the 95% reference limit for the latter patients. The results suggest that IFN-gamma aggravates the anemia of beta(o)-thal/HbE by activating mononuclear phagocytes for destruction of red cells but not by inhibiting erythropoiesis. The elevated serum levels of TNF-alpha and IL-1 could contribute to complications of the disease, such as cachexia and thromboembolic phenomena. |
| Databáze: | OpenAIRE |
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