Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane

Autor: Eduardo Alves, Alexandre Oliveira Ferreira, R. Lanna Filho, R. M. de Souza, J. L. de Azevedo, Maria Carolina Quecine
Přispěvatelé: Roberto Lanna Filho, UFRGS, Ricardo Magela de Souza, UFLA, ANDERSON FERREIRA, CPAMT, Maria Carolina Quecine Verdi, USP-ESALQ, Eduardo Alves, UFLA, USP-ESALQ.
Rok vydání: 2013
Předmět:
Zdroj: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
ISSN: 1448-6032
0815-3191
DOI: 10.1007/s13313-013-0233-z
Popis: We report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacterium Pseudomonas syringae pv. tomato strain NS4 transformed with the GFP expressing gene. P. s. pv. tomato strain NS4 was obtained from the transformation of P. s. pv. tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromo- somal integration. The GFP-marked strain was tested for hy- persensitivity and pathogenicity, as well as population studies onthephylloplane,todetermineitsepidemiologyandsurvival. In all of the bioassays strain NS4 presented similar character- istics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were pre- inoculated with the endophytic bacteria 4 days prior to inocu- lation with P. s. pv. tomato strains. On the tomato phylloplane the P. s. pv. tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced num- bers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the biocontrol of bacterial speck when applied as a foliar spray on the leaves. In addition, we highlighted the use of GFP-marked strain NS4 as a model system to study biocontrol agent and pathogen interactions, and growth and development of the pathogen on the tomato leaf surface.
Databáze: OpenAIRE
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