Development and characterization of 33 SNP markers in a critically endangered Hynobius amjiensis using ddRAD sequencing

Autor:	Xin Zhao, Yu Wang, Chen Shao, Guiming Liu, Xia Kan, Guoliang Wang, Shuangshuang Shan, Lili Zhang
Rok vydání:	2021
Předmět:	Genetics education.field_of_study Population Single-nucleotide polymorphism Locus (genetics) Biology biology.organism_classification Minor allele frequency Loss of heterozygosity Critically endangered Hynobius education Inbreeding Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 13:425-428
ISSN:	1877-7260 1877-7252
Popis:	Hynobius amjiensis was considered as one of the 29 most threatened amphibian in China. To effectively conserve, manage and recover the population of this critically endangered species, 33 single nucleotide polymorphism (SNP) markers were developed using double digest restriction-site associated DNA (ddRAD) sequencing. The minor allele frequency per locus ranged from 0.0161 to 0.5000. The observed heterozygosity and expected heterozygosity varied from 0.0323 to 0.6667 (average 0.3303) and from 0.0317 to 0.5000 (average 0.2772), respectively. The inbreeding coefficient value ranged between − 0.3315 and 0.0000. No significant deviation from Hardy-Weinberg equlibrium (P > 0.05) were found in all loci. These novel SNPs will be helpful for the population genetic assessment and conservation of H. amjiensis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8214a9ebd86a69ee3c919efd7264b9fd https://doi.org/10.1007/s12686-021-01228-1 Zobrazit plný text záznamu Full text from SpringerLink