SERODIAGNOSIS OF HUMAN LEPTOSPIROSIS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
| Autor: | Rachida Hassikou, Zakaria Mennane, Réda Charof, Aicha Qasmaoui, Hafida Oukoochood, Chahrazad Ouanaim, Waleed Al orry, Moustapha Arahou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification lcsh:R5-920 business.industry 030106 microbiology General Medicine medicine.disease Virology Leptospirosis Zoonosis 03 medical and health sciences Morocco Enzyme chemistry Slide Agglutination Test Medicine SAT ELISA business lcsh:Medicine (General) |
| Zdroj: | Australasian Medical Journal, Vol 10, Iss 1, Pp 30-34 (2017) |
| ISSN: | 1836-1935 |
| Popis: | Background Leptospirosis is a zoonotic bacterial disease that affects humans and animals. The disease occurs by contact direct or indirect with the urine of infected animal and common among agriculture workers, garbage collectors, sewage works, forestry and animal slaughtering. The disease also spreads in tropical regions where the conditions are favourable for leptospires. Aims The purpose of this study is to determine human leptospirosis among suspected cases by ELISA IgM (a retrospective study on 50 cases from 2004 to 2010). Methods Sera from patients had fever and jaundice suspected clinically from leptospirosis, were referred to the National Institute of Hygiene in Rabat, Morocco. ELISA IgM and SAT were used for the diagnosis. Results While 33 serums were positive by Slide Agglutination Test (SAT), thirty one serums were positive by ELISA IgM. The sensitivity was 62 per cent and 66 per cent in ELISA and SAT respectively. Conclusion ELISA IgM and SAT seem to be useful for human leptospirosis; they can detect the disease from the 5th day of the illness. They are easy, inexpensive and useful for developing countries and less equipped laboratories. However, if the first sera was negative, the second sera should obtained in the second week, or PCR used in combination with serological tests if it is available. |
| Databáze: | OpenAIRE |
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