Zwitterionic Polymers towards the Development of Orientation-Sensitive Bioprobes
| Autor: | Zijun Wang, Fanny Delille, Sophie Bartier, Thomas Pons, Nicolas Lequeux, Bruno Louis, Jongwook Kim, Thierry Gacoin |
|---|---|
| Přispěvatelé: | Laboratoire de physique de la matière condensée (LPMC), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et d'Etude des Matériaux (UMR 8213) (LPEM), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), IMRB - 'Biomechanics and Respiratory Apparatus' [Créteil] (U955 Inserm - UPEC), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Louis, Bruno |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
polarization
lanthanide Polymers [SDV]Life Sciences [q-bio] zwitterion Surfaces and Interfaces Biosensing Techniques Condensed Matter Physics Lanthanoid Series Elements nanorod [SDV] Life Sciences [q-bio] bioapplication Quantum Dots Electrochemistry functionalization General Materials Science Salts Spectroscopy |
| Zdroj: | Langmuir Langmuir, 2022, 38 (34), pp.10512-10519. ⟨10.1021/acs.langmuir.2c01286⟩ |
| ISSN: | 0743-7463 1520-5827 |
| Popis: | International audience; Dynamics with an orientational degree of freedom are fundamental in biological events. Probes with polarized luminescence enable a determination of the orientation. Lanthanide-doped nanocrystals can provide more precise analysis than quantum dots due to the non-photoblinking/bleaching nature and the multiple line-shaped emission. However, the intrinsic polarization property of the original nanocrystals often deteriorates in complex physiological environments, because the colloidal stability easily breaks and the probes aggregate in the media with abundant salts and macromolecules. Engineering the surface chemistry of the probes is thus essential to be compatible with biosystems, which has remained a challenging task that should be exclusively addressed for each specific probe. Here, we demonstrate a facile and efficient surface functionalization of lanthanide-doped nanorods by zwitterionic block copolymers. Due to the steric interaction and the intrinsic zwitterionic nature of the polymers, high colloidal stability of the zwitterionic nanorod suspension is achieved over wide ranges of pH and concentration of salts, even giving rise to the lyotropic liquid crystalline behavior of the nanorods in physiological media. The shear-aligned ability is shown to be unaltered by the coated polymers and thus the strongly polarized emission of Eu 3+ is preserved. Besides, biological experiments reveal good biocompatibility of the zwitterionic nanorods with negligible non-specific binding. This study is a stepping stone for the use of the nanorods as orientation probes in biofluids and validates the strategy of coupling zwitterions to lanthanide-doped nanocrystals for various bio-applications. |
| Databáze: | OpenAIRE |
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