Physiological effect of protein kinase C on ENaC-mediated lung liquid regulation in the adult rat lung
| Autor: | Dafydd V. Walters, A Benjamin, Benjamin Soukup, M Orogo-Wenn |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Epithelial sodium channel medicine.medical_specialty Indoles Physiology Pulmonary function testing Amiloride Maleimides chemistry.chemical_compound Physiology (medical) Internal medicine Electric Impedance Terbutaline medicine Animals Enzyme Inhibitors Rats Wistar Epithelial Sodium Channels Lung Cells Cultured Protein Kinase C Protein kinase C Benzophenanthridines Chemistry Ionomycin Biological Transport Epithelial Cells Cell Biology PKC activation Rats Ringer's Solution medicine.anatomical_structure Endocrinology Tetradecanoylphorbol Acetate Isotonic Solutions Signal Transduction Sodium Channel Blockers |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 302:L133-L139 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00031.2011 |
| Popis: | Tight control of lung liquid (LL) regulation is vital for pulmonary function. The aim of this work was to determine whether PKC activation is involved in the physiological regulation of LL volume in a whole lung preparation. Rat lungs were perfused with a modified Ringer solution, and the lumen was filled with the same solution without glucose. LL volume was measured during a control period and after modulating drugs were administered, and net LL transepithelial movement ( Jv) was calculated. When the PKC activator PMA (10−5 M) and the Ca2+ ionophore ionomycin (10−6 M) were instilled into the lung together, Jv was significantly reduced ( P = 0.03). This reduction was blocked by the PKC inhibitor chelerythrine chloride (10−6 M; P = 0.56) and by a second PKC inhibitor GF109203X (10−5 M; P = 0.98). When PMA and ionomycin were added with the β-adrenergic agonist terbutaline, the terbutaline-induced increase in Jv was abolished. Addition of PMA and ionomycin with the epithelial Na+ channel (ENaC) blocker amiloride had no additional inhibitory effect. Together, these results suggest that PKC is likely to be involved in LL absorption, and the ability of PMA/ionomycin to block the terbutaline-induced increase in Jv suggests that the downstream target of PKC is ENaC. |
| Databáze: | OpenAIRE |
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