The light chain subunit is required for clathrin function in Saccharomyces cerevisiae
| Autor: | Diana S. Chu, Babak Pishvaee, Gregory S. Payne |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Multiprotein complex
Macromolecular Substances Protein subunit Saccharomyces cerevisiae Coated Vesicles Immunoglobulin light chain Endocytosis Biochemistry Clathrin Fungal Proteins Molecular Biology biology Vesicle Cell Membrane Wild type Biological Transport Cell Biology Intracellular Membranes biology.organism_classification Molecular biology Cell biology Cell Compartmentation Mutagenesis Insertional biology.protein Mating Factor Peptides |
| Zdroj: | The Journal of biological chemistry. 271(51) |
| ISSN: | 0021-9258 |
| Popis: | Clathrin, a multimeric protein involved in intracellular protein trafficking, is composed of three heavy chains (Chc) and three light chains (Clc). Upon disruption (clc1Delta) of the single Clc-encoding gene (CLC1) in yeast, the steady state protein levels of Chc decreased 5-10-fold compared with wild type cells; consequently, phenotypes exhibited by clc1Delta cells may result indirectly from the loss of Chc as opposed to the absence of Clc. As an approach to directly examine Clc function, clc1Delta strains were generated that carry a multicopy plasmid containing the clathrin heavy chain gene (CHC1), resulting in levels of Chc 5-10-fold elevated over wild-type levels. As with deletion of CHC1, deletion of CLC1 results in defects in growth, receptor-mediated endocytosis, and maturation of the mating pheromone alpha-factor. However, elevated Chc expression in clc1Delta cells partially suppresses the growth and alpha-factor maturation defects displayed by clc1Delta cells alone. Biochemical analyses indicate that trimerization and assembly of Chc are perturbed in the absence of Clc, resulting in vesiculation defects. Our results demonstrate that the light chain subunit of clathrin is required for efficient Chc trimerization, proper formation of clathrin coats, and the generation of clathrin-coated vesicles. |
| Databáze: | OpenAIRE |
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