The cell-penetrating peptide, Pep-1, has activity against intracellular chlamydial growth but not extracellular forms of Chlamydia trachomatis
| Autor: | Pete Chandrangsu, Ming Tan, Johnny C. Akers, Kinrin Yamanaka, Michael E. Selsted, Dat Q. Tran, Jessica Cruz de Leon, Narae Park, Naomi S. Morrissette |
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| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Cytoplasm Staphylococcus aureus Cysteamine education Colony Count Microbial Chlamydiae Chlamydia trachomatis Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology Mice Microscopy Electron Transmission Escherichia coli medicine Extracellular Animals Pharmacology (medical) Chlamydiaceae Cells Cultured Original Research Inclusion Bodies Pharmacology Chlamydia Intracellular parasite biology.organism_classification medicine.disease Virology Anti-Bacterial Agents Infectious Diseases Chlamydiales cardiovascular system Peptides Toxoplasma Intracellular |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 63:115-123 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkn436 |
| Popis: | Objectives: In the course of studies to identify novel treatment strategies against the pathogenic bacterium, Chlamydia, we tested the carrier peptide, Pep-1, for activity against an intracellular infection. Methods: Using a cell culture model of Chlamydia trachomatis infection, the effect of Pep-1 was measured by incubating the peptide with extracellular chlamydiae prior to infection, or by adding Pep-1 to the medium at varying times after infection, and assaying for inhibition of inclusion formation. Results: Pep-1 had a concentration-dependent effect on chlamydial growth with 100% inhibition of inclusion formation at 8 mg/L peptide. There was a window of susceptibility during the chlamydial developmental cycle with a maximal effect when treatment was begun within 12 h of infection. Pep-1 treatment caused a severe reduction in the production of infectious progeny even when started later, when the effect on inclusion formation was minimal. Furthermore, electron micrographs showed a paucity of progeny elementary bodies (EBs) in the inclusion. In contrast, pre-incubation of EBs with Pep-1 prior to infection did not affect inclusion formation. Taken together, these findings indicate that the antichlamydial effect was specific for the intracellular stage of chlamydial infection. By comparison, Pep-1 had no antimicrobial activity against Escherichia coli and Staphylococcus aureus or the obligate intracellular parasite, Toxoplasma gondii. Conclusions: Pep-1 has antichlamydial activity by preventing intracellular chlamydial growth and replication but has no effect on extracellular chlamydiae. |
| Databáze: | OpenAIRE |
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