Comparative genome sequence analysis of several species in the genus
| Autor: | Luciana Albuquerque, Nadine Castelhano, Pedro Raposo, Hugo J. C. Froufe, Igor Tiago, Rita Severino, Inês Roxo, Inês Gregório, Cristina Barroso, Conceição Egas, Milton S. da Costa |
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| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial New Taxa Microbiology Hot Springs 03 medical and health sciences Taxonomic Description RNA Ribosomal 16S Proteobacteria Ecology Evolution Behavior and Systematics Burkholderiales Phylogeny 030304 developmental biology 0303 health sciences Base Composition Comparative Genomic Hybridization Genome Tepidimonas charontis sp. nov Portugal 030306 microbiology Fatty Acids Betaproteobacteria Nucleic Acid Hybridization General Medicine Sequence Analysis DNA Bacterial Typing Techniques Water Microbiology Corrigendum |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology |
| ISSN: | 1466-5034 |
| Popis: | We performed high-quality genome sequencing of eight strains of the species of the genus Tepidimonas and examined the genomes of closely related strains from the databases to understand why Tepidimonas taiwanensis is the only strain of this genus that utilizes glucose and fructose for growth. We found that the assimilation of these hexoses by T. taiwanensis was due to the presence of two transporters that are absent in all other genomes of strains of members of the genus Tepidimonas examined. Some strains lack genes coding for glucokinase, but the Embden–Meyerhof–Parnas pathway appears to be otherwise complete. The pentose phosphate pathway has a complete set of genes, but genes of the Entner–Doudoroff pathway were not identified in the genomes of any of the strains examined. Genome analysis using average nucleotide identity (ANIb), digital DNA–DNA hybridization (dDDH), average amino acid identity (AAI) and phylogenetic analysis of 400 conserved genes was performed to assess the taxonomic classification of the organisms. Two isolates of the genus Tepidimonas from the hot spring at São Pedro do Sul, Portugal, designated SPSP-6T and SPSPC-18 were also examined in this study. These organisms are mixotrophic, have an optimum growth temperature of about 50 ºC, utilize several organic acids and amino acids for growth but do not grow on sugars. Distinctive phenotypic, 16S rRNA gene sequence and genomic characteristics of strains SPSP-6T and SPSPC-18 lead us to propose a novel species based on strain SPSP-6T for which we recommend the name Tepidimonas charontis sp. nov. (=CECT 9683T=LMG 30884T). |
| Databáze: | OpenAIRE |
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