The chemical composition of the membranes of protoplasts and L-forms of Staphylococcus
| Autor: | J. B. Ward, H. R. Perkins |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Genetics
Microbial History Chromatography Gas Staphylococcus Carbohydrates L Forms Phospholipid Biology Education Cell wall chemistry.chemical_compound Glycolipid Ultrasonics Amino Acids Alanine Phosphatidylglycerol Chromatography Autoanalysis Protoplasts Cell Membrane Fatty Acids Articles Silicon Dioxide biology.organism_classification Lipids Computer Science Applications RNA Bacterial Membrane Biochemistry chemistry lipids (amino acids peptides and proteins) Streptomyces griseus Bacteria |
| Zdroj: | Biochemical Journal. 106:391-400 |
| ISSN: | 0306-3283 |
| DOI: | 10.1042/bj1060391 |
| Popis: | Membrane fractions were prepared from Staphylococcus aureus H and 100 after dissolution of the cell walls by a lytic enzyme from Streptomyces griseus. Membranes were also prepared from the L-forms derived from the same strains. The membranes were analysed for protein, lipid, carbohydrate and RNA contents, and the fatty acid composition of the lipids was determined. A branched-chain saturated C15 acid was the major component in all samples, and the correspondence between L-forms and parent bacteria was fairly close. The lipids were separated into non-polar-lipid, glycolipid and phospholipid fractions; the L-forms contained a little more neutral lipid and much more glycolipid than the parent bacteria. In all membranes the glycolipid, which accounted for all the carbohydrate present, was a diglucosyl diglyceride. The major phospholipids of the protoplast membranes were phosphatidylglycerol and some lipoamino acids (lysine and a little alanine). On the other hand, diphosphatidylglycerol was the chief phospholipid found in L-form membranes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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