First Detection of the Antiseptic Resistance GeneqacA/B inEnterococcus faecalis
| Autor: | Christina Hölzel, Meike Bischoff, Johann Bauer, Petra Preikschat, Karin Schwaiger, Gabriele Mölle |
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| Rok vydání: | 2012 |
| Předmět: |
Serum
Microbiology (medical) medicine.drug_class Immunology Antibiotics Microbial Sensitivity Tests Polymerase Chain Reaction Microbiology Enterococcus faecalis Feces Antiseptic Drug Resistance Bacterial medicine Animals Humans Gene Gram-Positive Bacterial Infections Pharmacology biology Nosocomial pathogens Genus Enterococcus biology.organism_classification Anti-Bacterial Agents Bacterial Typing Techniques Meat Products Genes Bacterial Mutation Anti-Infective Agents Local Cattle Dairy Products Bacteria |
| Zdroj: | Microbial Drug Resistance. 18:7-12 |
| ISSN: | 1931-8448 1076-6294 |
| Popis: | Resistance to disinfectants is well investigated in staphylococci and pseudomonads but nearly unexplored in bacteria of the genus Enterococcus, despite their rising significance as nosocomial pathogens. In this study, Enterococcus faecalis (n=585) from blood (n=42) and stool (n=109) of hospitalized humans, from faeces of farm animals (n=226), and from food (milk and dairy products, n=96; meat and meat products, n=112) were screened for the presence of qac-genes (qacA, qacB, qacC, smr [qacC+qacD], qacEΔ1, qacG, qacH, qacJ) via PCR. The isolates' susceptibility to a quaternary ammonium compound (didecyldimethylammoniumchloride, DDAC) and antibiotics was assessed by microdilution. Four E. faecalis strains were positive for qac-genes: qacA/B was found in one isolate from cattle and one isolate from human blood; smr (qacC+qacD) was detected in one isolate from human stool and in one isolate from cheese ("Camembert"). The sequences of the qacA/B-amplicons differed in two basepairs. DDAC had an elevated minimum inhibitory concentration (MIC) of 2.45-3.5 mg/L in one qacA/B-positive strain from human blood, whereas the other qac-gene carriers had wild-type MIC-values for DDAC (1.05 mg/L). This is the first detection of qacA/B in the genus Enterococcus. |
| Databáze: | OpenAIRE |
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