Caspase-6 Induces 7A6 Antigen Localization to Mitochondria During FAS-induced Apoptosis of Jurkat Cells
| Autor: | Yukitoshi Nagahara, Hiroaki Suita, Takahisa Shinomiya |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Blotting Western Apoptosis Caspase 6 Mitochondrion Jurkat cells Flow cytometry 03 medical and health sciences Jurkat Cells Western blot medicine Humans fas Receptor Caspase medicine.diagnostic_test biology Chemistry Membrane Proteins General Medicine Flow Cytometry Caspase Inhibitors Cell biology Mitochondria Cytosol 030104 developmental biology Oncology biology.protein |
| Zdroj: | Anticancer research. 37(4) |
| ISSN: | 1791-7530 |
| Popis: | Background Mitochondria are central to apoptosis. However, apoptosis progression involving mitochondria is not fully understood. A factor involved in mitochondria-mediated apoptosis is 7A6 antigen. 7A6 localizes to mitochondria from the cytosol during apoptosis, which seems to involve 'effector' caspases. In this study, we investigated the precise role of effector caspases in 7A6 localization to mitochondria during apoptosis. Materials and methods Human T-cell lymphoma Jurkat cells were treated with an antibody against FAS. 7A6 localization was analyzed by confocal laser scanning microscopy and flow cytometry. Caspases activation was determined by western blot analysis. Results 7A6 localization to mitochondria during anti-FAS-induced apoptosis was significantly reduced by the caspase-6 inhibitor, N-acetyl-Val-Glu-Ile-Asp-aldehyde, but not by the caspase-3 inhibitor, N-acetyl-Asp-Asn-Leu-Asp-aldehyde, nor caspase-7/3 inhibitor, N-acetyl-Asp-Gln-Thr-Asp-aldehyde. Moreover, caspase-6 down-regulation suppressed 7A6 localization to mitochondria. Conclusion Caspase-6 regulates 7A6 localization to mitochondria during anti-FAS-induced apoptosis of Jurkat cells. |
| Databáze: | OpenAIRE |
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