Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
| Autor: | Pankaj Kumar Chauhan, S. Nishad, Anu Ghosh, Ramanathan Sowdhamini |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
0301 basic medicine Cell biology Radiobiology DNA Repair DNA repair Science India Computational biology Biology Biochemistry Article 03 medical and health sciences 0302 clinical medicine Radiation Ionizing Background Radiation Humans Protein Interaction Maps HSPA8 Heat-Shock Proteins Multidisciplinary Natural hazards Environmental Exposure Chromatin PRKACA 030104 developmental biology 14-3-3 Proteins 030220 oncology & carcinogenesis YWHAZ Leukocytes Mononuclear Medicine Phosphorylation Biomarkers |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Understanding exposures to low doses of ionizing radiation are relevant since most environmental, diagnostic radiology and occupational exposures lie in this region. However, the molecular mechanisms that drive cellular responses at these doses, and the subsequent health outcomes, remain unclear. A local monazite-rich high level natural radiation area (HLNRA) in the state of Kerala on the south-west coast of Indian subcontinent show radiation doses extending from ≤ 1 to ≥ 45 mGy/y and thus, serve as a model resource to understand low dose mechanisms directly on healthy humans. We performed quantitative discovery proteomics based on multiplexed isobaric tags (iTRAQ) coupled with LC–MS/MS on human peripheral blood mononuclear cells from HLNRA individuals. Several proteins involved in diverse biological processes such as DNA repair, RNA processing, chromatin modifications and cytoskeletal organization showed distinct expression in HLNRA individuals, suggestive of both recovery and adaptation to low dose radiation. In protein–protein interaction (PPI) networks, YWHAZ (14-3-3ζ) emerged as the top-most hub protein that may direct phosphorylation driven pro-survival cellular processes against radiation stress. PPI networks also identified an integral role for the cytoskeletal protein ACTB, signaling protein PRKACA; and the molecular chaperone HSPA8. The data will allow better integration of radiation biology and epidemiology for risk assessment [Data are available via ProteomeXchange with identifier PXD022380]. |
| Databáze: | OpenAIRE |
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